Abstract
Endurance exercise is known to cause a rise in serum creatinine. It is not known to what extent this rise reflects renal stress and a potential acute kidney injury (AKI). Increases in Insulin Like Growth Factor Binding Protein 7 (IGFBP7) and Tissue Inhibitor of Metalloprotinases-2 (TIMP-2), urinary biomarkers of cell cycle arrest and renal stress, are associated with the development of AKI.
Design
Repeated measures study
Methods
Runners were recruited at the 2019 Brighton Marathon (UK) and provided urine and blood samples at baseline, immediately post-race and 24hrs post-race. Serum creatinine, urinary creatinine and urinary IGFBP7 and TIMP-2 were analysed from the samples.
Results
Seventy nine participants (23 females, 56 males), aged 43 ± 10yrs (mean ± SD), finish time 243 ± 40mins were included for analysis. Serum creatinine increased over the race by 40 ± 26% (p<0.001), TIMP-2 increased by 555 ± 697% (p<0.001) and IGFBP7 increased by 1094 ± 1491% (p<0.001) over the race. A subset of twenty-two participants supplied samples 24 hours post-race, reporting values similar to baseline at for all variables by this time. Significant increases (p<0.01) were seen in markers when corrected for urinary creatinine.
Conclusions
This study is the first to report large rises in IGFBP7 and TIMP-2 following marathon running. This suggests that rises in creatinine are not fully explained by changes in production and clearance and may reflect a state of kidney stress, or injury.
Design
Repeated measures study
Methods
Runners were recruited at the 2019 Brighton Marathon (UK) and provided urine and blood samples at baseline, immediately post-race and 24hrs post-race. Serum creatinine, urinary creatinine and urinary IGFBP7 and TIMP-2 were analysed from the samples.
Results
Seventy nine participants (23 females, 56 males), aged 43 ± 10yrs (mean ± SD), finish time 243 ± 40mins were included for analysis. Serum creatinine increased over the race by 40 ± 26% (p<0.001), TIMP-2 increased by 555 ± 697% (p<0.001) and IGFBP7 increased by 1094 ± 1491% (p<0.001) over the race. A subset of twenty-two participants supplied samples 24 hours post-race, reporting values similar to baseline at for all variables by this time. Significant increases (p<0.01) were seen in markers when corrected for urinary creatinine.
Conclusions
This study is the first to report large rises in IGFBP7 and TIMP-2 following marathon running. This suggests that rises in creatinine are not fully explained by changes in production and clearance and may reflect a state of kidney stress, or injury.
Original language | English |
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Pages (from-to) | 14-18 |
Number of pages | 5 |
Journal | Journal of Science and Medicine in Sport |
Volume | 26 |
Issue number | 1 |
DOIs | |
Publication status | Published - 24 Oct 2022 |
Bibliographical note
Funding Information:This study was funded by the British Association of Sport and Exercise Medicine 2019 research bursary. The bursary paid for the equipment and consumables required to collect the blood and urine samples. The processing and measurement of the biomarkers was performed free of charge by Ortho Clinical Diagnostics (Felindre Meadows, Pencoed, Bridgend CF35 5PZ). Samples were provided to Ortho Clinical Diagnostics following sample anonymisation and Ortho Clinical Diagnostics had no role in study design, data collection or manuscript writing.
Funding Information:
Thanks go to the Brighton marathon organisers, medical team and of course the willing runners. The project was funded by the British Association of Sport and Exercise Medicine (BASEM). We are grateful for the support of Ortho Clinical Diagnostics (OCD), Felindre Meadows, Pencoed, Bridgend CF35 5PZ for the measurements of TIMP-2 and IGFBP-7 without which this study would not have been possible. Neither BASEM nor OCD were involved in study design, data analysis or manuscript production.
Publisher Copyright:
© 2022 Elsevier Ltd
Keywords
- Biomarker
- Endurance
- IGFBP7
- Running
- TIMP-2
- Acute kidney injury