to find new sources of L-asparaginase produced by eukaryotic microorganisms with low glutaminase activity. This work aimed to identify the L-asparaginase gene sequence from Penicillium sizovae, a filamentous fungus isolated from the Brazilian Savanna (Cerrado) soil with low glutaminase activity, and to biosynthesize higher yields of this enzyme in the yeast Komagataella phaffii. The L-asparaginase gene sequence of P. sizovae was identified by homology to L-asparaginases from species of Penicillium of the section Citrina: P. citrinum and P. steckii. Partial L-asparaginase from P. sizovae, lacking the periplasmic signaling sequence, was cloned, and expressed intracellularly with highest enzymatic activity achieved by a MUT+ clone cultured in BMM expression medium; a value 5-fold greater than that obtained by native L-asparaginase in P. sizovae cells. To the best of our knowledge, this is the first literature report of the heterologous production of an L-asparaginase from a filamentous fungus by a yeast.
|Number of pages||17|
|Publication status||Published - 14 Jun 2022|
Bibliographical noteFunding Information:
Funding: This research was partially funded by the Rising Stars Initiative, University of Brighton, United Kingdom; by the Higher Education Personnel Improvement Coordination (CAPES) [Finance Code 001] and the Foundation for Research Support of the Federal District (FAP-DF) [process numbers 193.001.661/2017, 0193.001797/2017 and 193.0000.919/2020-07].
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