Isolation and Generation of Osteoblasts

IP Perpétuo; LE Bourne; IR Orriss

doi:10.1007/978-1-4939-8997-3_2

Isolation and Generation of Osteoblasts

IP Perpétuo, LE Bourne, IR Orriss

School of Applied Sciences

Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Chapter › peer-review

Abstract

This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.

Original language	English
Title of host publication	Bone Research Protocols
Editors	Aymen I. Idris
Publisher	Humana Press
Pages	21–38
Volume	1914
ISBN (Electronic)	9781493989973
ISBN (Print)	9781493989966
DOIs	https://doi.org/10.1007/978-1-4939-8997-3_2
Publication status	Published - 8 Feb 2019

Publication series

Name	Methods in molecular biology (Clifton, N.J.)

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10.1007/978-1-4939-8997-3_2

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AB - This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.

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U2 - 10.1007/978-1-4939-8997-3_2

DO - 10.1007/978-1-4939-8997-3_2

M3 - Chapter

C2 - 30729458

SN - 9781493989966

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T3 - Methods in molecular biology (Clifton, N.J.)

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PB - Humana Press

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Isolation and Generation of Osteoblasts

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