This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.
|Title of host publication||Bone Research Protocols|
|Editors||Aymen I. Idris|
|Publication status||Published - 8 Feb 2019|
|Name||Methods in molecular biology (Clifton, N.J.)|