Isolation and Generation of Osteoblasts

IP Perpétuo, LE Bourne, IR Orriss

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNChapterpeer-review


This chapter describes the isolation, culture, and staining of osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. An additional focus of this chapter will be the culture of osteoblasts from less conventional animal species.
Original languageEnglish
Title of host publicationBone Research Protocols
EditorsAymen I. Idris
PublisherHumana Press
ISBN (Electronic)9781493989973
ISBN (Print)9781493989966
Publication statusPublished - 8 Feb 2019

Publication series

NameMethods in molecular biology (Clifton, N.J.)


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