Isolation and Culture of Osteoblasts

Lucie E Bourne; Isabel R Orriss

doi:10.1007/978-1-0716-4306-8_1

Isolation and Culture of Osteoblasts

Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Chapter › peer-review

Abstract

This chapter describes the isolation, culture, and staining of primary osteoblasts. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralization, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. While the focus is on rodent and human osteoblasts, this chapter will also cover the culture of osteoblasts from less conventional animal species.

Original language	English
Title of host publication	Bone Research Protocols
Editors	Aymen I. Idris
Place of Publication	New York
Publisher	Humana Press
Pages	3-22
Number of pages	20
Volume	2885
ISBN (Print)	9781071643051
DOIs	https://doi.org/10.1007/978-1-0716-4306-8_1
Publication status	Published - 1 Jun 2025

Publication series

Name	Methods in molecular biology
Publisher	Springer
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.

Keywords

Rats
Cell Culture Techniques - methods
Osteoblast
Animals
Cell Differentiation
Cell Separation - methods
Osteoblasts - cytology - metabolism
Mineralization
Humans
Mice
Bone formation
Cells, Cultured

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10.1007/978-1-0716-4306-8_1

Cite this

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AU - Orriss, Isabel R

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KW - Cell Differentiation

KW - Cell Separation - methods

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KW - Mice

KW - Bone formation

KW - Cells, Cultured

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Isolation and Culture of Osteoblasts

Abstract

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Keywords

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