Intersex related gene expression profiles in clams Scrobicularia plana: molecular markers and environmental application

Corina Ciocan, E. Cubero-Leon, W.J. Langston, N. Pope, Keith Cornelius, E.M. Hill, Diana Alvarez-Munoz, Paolo Indiveri, Adelaide Lerebours, C. Minier, J.M. Rotchell

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Intersex, the appearance of female characteristics in male gonads, has been identified in several aquatic species. It is a widespread phenomenon in populations of the bivalve, Scrobicularia plana, from the southwest coast of the U.K. Genes previously identified as differentially expressed (ferritin, testicular haploid expressed gene, THEG, proliferating cell nuclear antigen, PCNA; receptor activated protein kinase C, RACK; cytochrome B, CYB; and cytochrome c oxidase 1, COX1) in intersex clams relative to normal male clams, were selected for characterisation and an environmental survey of the Channel region. Transcripts were significantly differentially expressed at sites with varying intersex incidence and contaminant burdens. Significant correlations between specific gene expressions, key contaminants and sampling locations have been identified, though no single gene was associated with intersex incidence. The results highlight the difficulty in understanding the intersex phenomenon in molluscs where there is still a lack of knowledge on the control of normal reproduction.
Original languageEnglish
Pages (from-to)610-617
Number of pages8
JournalMarine Pollution Bulletin
Volume95
Issue number2
DOIs
Publication statusPublished - 5 Mar 2015

Fingerprint

clams
gene expression
genetic markers
incidence
proliferating cell nuclear antigen
genes
protein kinase C
ferritin
cytochromes
haploidy
cytochrome-c oxidase
molluscs
gonads
Bivalvia
coasts
receptors
sampling

Bibliographical note

Copyright © 2015 Elsevier B.V. or its licensors or contributors.

Keywords

  • Intersex
  • Clams
  • English Channel
  • Gene expression

Cite this

Ciocan, Corina ; Cubero-Leon, E. ; Langston, W.J. ; Pope, N. ; Cornelius, Keith ; Hill, E.M. ; Alvarez-Munoz, Diana ; Indiveri, Paolo ; Lerebours, Adelaide ; Minier, C. ; Rotchell, J.M. / Intersex related gene expression profiles in clams Scrobicularia plana: molecular markers and environmental application. In: Marine Pollution Bulletin. 2015 ; Vol. 95, No. 2. pp. 610-617.
@article{9ab77d7cb56340ab963a152b5d110a14,
title = "Intersex related gene expression profiles in clams Scrobicularia plana: molecular markers and environmental application",
abstract = "Intersex, the appearance of female characteristics in male gonads, has been identified in several aquatic species. It is a widespread phenomenon in populations of the bivalve, Scrobicularia plana, from the southwest coast of the U.K. Genes previously identified as differentially expressed (ferritin, testicular haploid expressed gene, THEG, proliferating cell nuclear antigen, PCNA; receptor activated protein kinase C, RACK; cytochrome B, CYB; and cytochrome c oxidase 1, COX1) in intersex clams relative to normal male clams, were selected for characterisation and an environmental survey of the Channel region. Transcripts were significantly differentially expressed at sites with varying intersex incidence and contaminant burdens. Significant correlations between specific gene expressions, key contaminants and sampling locations have been identified, though no single gene was associated with intersex incidence. The results highlight the difficulty in understanding the intersex phenomenon in molluscs where there is still a lack of knowledge on the control of normal reproduction.",
keywords = "Intersex, Clams, English Channel, Gene expression",
author = "Corina Ciocan and E. Cubero-Leon and W.J. Langston and N. Pope and Keith Cornelius and E.M. Hill and Diana Alvarez-Munoz and Paolo Indiveri and Adelaide Lerebours and C. Minier and J.M. Rotchell",
note = "Copyright {\circledC} 2015 Elsevier B.V. or its licensors or contributors.",
year = "2015",
month = "3",
day = "5",
doi = "10.1016/j.marpolbul.2015.02.019",
language = "English",
volume = "95",
pages = "610--617",
journal = "Marine Pollution Bulletin",
issn = "0025-326X",
number = "2",

}

Ciocan, C, Cubero-Leon, E, Langston, WJ, Pope, N, Cornelius, K, Hill, EM, Alvarez-Munoz, D, Indiveri, P, Lerebours, A, Minier, C & Rotchell, JM 2015, 'Intersex related gene expression profiles in clams Scrobicularia plana: molecular markers and environmental application', Marine Pollution Bulletin, vol. 95, no. 2, pp. 610-617. https://doi.org/10.1016/j.marpolbul.2015.02.019

Intersex related gene expression profiles in clams Scrobicularia plana: molecular markers and environmental application. / Ciocan, Corina; Cubero-Leon, E.; Langston, W.J.; Pope, N.; Cornelius, Keith; Hill, E.M.; Alvarez-Munoz, Diana; Indiveri, Paolo; Lerebours, Adelaide; Minier, C.; Rotchell, J.M.

In: Marine Pollution Bulletin, Vol. 95, No. 2, 05.03.2015, p. 610-617.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Intersex related gene expression profiles in clams Scrobicularia plana: molecular markers and environmental application

AU - Ciocan, Corina

AU - Cubero-Leon, E.

AU - Langston, W.J.

AU - Pope, N.

AU - Cornelius, Keith

AU - Hill, E.M.

AU - Alvarez-Munoz, Diana

AU - Indiveri, Paolo

AU - Lerebours, Adelaide

AU - Minier, C.

AU - Rotchell, J.M.

N1 - Copyright © 2015 Elsevier B.V. or its licensors or contributors.

PY - 2015/3/5

Y1 - 2015/3/5

N2 - Intersex, the appearance of female characteristics in male gonads, has been identified in several aquatic species. It is a widespread phenomenon in populations of the bivalve, Scrobicularia plana, from the southwest coast of the U.K. Genes previously identified as differentially expressed (ferritin, testicular haploid expressed gene, THEG, proliferating cell nuclear antigen, PCNA; receptor activated protein kinase C, RACK; cytochrome B, CYB; and cytochrome c oxidase 1, COX1) in intersex clams relative to normal male clams, were selected for characterisation and an environmental survey of the Channel region. Transcripts were significantly differentially expressed at sites with varying intersex incidence and contaminant burdens. Significant correlations between specific gene expressions, key contaminants and sampling locations have been identified, though no single gene was associated with intersex incidence. The results highlight the difficulty in understanding the intersex phenomenon in molluscs where there is still a lack of knowledge on the control of normal reproduction.

AB - Intersex, the appearance of female characteristics in male gonads, has been identified in several aquatic species. It is a widespread phenomenon in populations of the bivalve, Scrobicularia plana, from the southwest coast of the U.K. Genes previously identified as differentially expressed (ferritin, testicular haploid expressed gene, THEG, proliferating cell nuclear antigen, PCNA; receptor activated protein kinase C, RACK; cytochrome B, CYB; and cytochrome c oxidase 1, COX1) in intersex clams relative to normal male clams, were selected for characterisation and an environmental survey of the Channel region. Transcripts were significantly differentially expressed at sites with varying intersex incidence and contaminant burdens. Significant correlations between specific gene expressions, key contaminants and sampling locations have been identified, though no single gene was associated with intersex incidence. The results highlight the difficulty in understanding the intersex phenomenon in molluscs where there is still a lack of knowledge on the control of normal reproduction.

KW - Intersex

KW - Clams

KW - English Channel

KW - Gene expression

U2 - 10.1016/j.marpolbul.2015.02.019

DO - 10.1016/j.marpolbul.2015.02.019

M3 - Article

VL - 95

SP - 610

EP - 617

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

SN - 0025-326X

IS - 2

ER -