Intersex in Scrobicularia plana: transcriptomic analysis reveals novel genes involved in endocrine disruption

Corina Ciocan, E. Cubero-Leon, Mika Peck, W.J. Langston, N. Pope, C. Minier, J.M. Rotchell

Research output: Contribution to journalArticle

Abstract

Intersex, the appearance of female characteristics in male gonads, has been identified in a wide range of aquatic species worldwide, yet the underpinning molecular etiology remains uncharacterised. The presence of intersex has been shown to be a widespread phenomenon in bivalve, S. plana, populations from the southwest coast of the U.K., as well as inducible in an experimental exposure regime using endocrine disrupting compounds (EDCs). Herein, we use the suppressive subtractive hybridisation approach to isolate differentially expressed transcripts in S. plana males exhibiting intersex. Transcripts involved in cell signalling, cell cycle control, energy production/metabolism, microtubule assembly, and sperm physiology, are all highlighted as differentially expressed in intersex male clams. These provide both an insight into the molecular mechanisms of action involved in the development of intersex, as well as facilitating potential molecular-level 'early warning' biomarkers of the condition
Original languageEnglish
Pages (from-to)12936-12942
Number of pages7
JournalEnvironmental Science and Technology
Volume46
Issue number23
DOIs
Publication statusPublished - 30 Oct 2012

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transcriptomics
suppression subtractive hybridization
endocrine-disrupting chemicals
genes
clams
microtubules
etiology
gonads
Bivalvia
cell cycle
mechanism of action
biomarkers
physiology
spermatozoa
coasts
metabolism
energy
cells

Cite this

Ciocan, Corina ; Cubero-Leon, E. ; Peck, Mika ; Langston, W.J. ; Pope, N. ; Minier, C. ; Rotchell, J.M. / Intersex in Scrobicularia plana: transcriptomic analysis reveals novel genes involved in endocrine disruption. In: Environmental Science and Technology. 2012 ; Vol. 46, No. 23. pp. 12936-12942.
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Intersex in Scrobicularia plana: transcriptomic analysis reveals novel genes involved in endocrine disruption. / Ciocan, Corina; Cubero-Leon, E.; Peck, Mika; Langston, W.J.; Pope, N.; Minier, C.; Rotchell, J.M.

In: Environmental Science and Technology, Vol. 46, No. 23, 30.10.2012, p. 12936-12942.

Research output: Contribution to journalArticle

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