Insight into the mechanism of galactokinase: role of a critical glutamate residue and helix/coil transitions

Margaret McAuley, Meilan Huang, David Timson

Research output: Contribution to journalArticle

Abstract

Galactokinase, the enzyme which catalyses the first committed step in the Leloir pathway, hasattracted interest due to its potential as a biocatalyst and as a possible drug target in the treatment of type I galactosemia. The mechanism of the enzyme is not fully elucidated. Molecular dynamics (MD) simulations of galactokinase with the active site residues Arg-37 and Asp-186 altered predicted that two regions (residues 174-179 and 231-240) had different dynamics as a consequence.Interestingly, the same two regions were also affected by alterations in Arg-105, Glu-174 and Arg-228. These three residues were identified as important in catalysis in previous computational studies on human galactokinase. Alteration of Arg-105 to methionine resulted in a modest reduction in activity with little change in stability. When Arg-228 was changed to methionine, the enzyme’s interaction with both ATP and galactose was affected. This variant was significantly less stable than the wild-type protein. Changing Glu-174 to glutamine (but not to aspartate) resulted in no detectable activity and a less stable enzyme. Overall, these combined in silico and in vitro studies demonstrate the importance of a negative charge at position 174 and highlight the critical role of the dynamics in to key regions of the protein. We postulate that these regions may be critical formediating the enzyme’s structure and function.
Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalBBA - Proteins and Proteomics
Volume1865
Issue number3
DOIs
Publication statusPublished - 24 Oct 2016

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Galactokinase
Glutamic Acid
Enzymes
Methionine
Galactosemias
Molecular Dynamics Simulation
Glutamine
Catalysis
Galactose
Aspartic Acid
Computer Simulation
Catalytic Domain
Proteins
Adenosine Triphosphate
Pharmaceutical Preparations

Bibliographical note

© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Cite this

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abstract = "Galactokinase, the enzyme which catalyses the first committed step in the Leloir pathway, hasattracted interest due to its potential as a biocatalyst and as a possible drug target in the treatment of type I galactosemia. The mechanism of the enzyme is not fully elucidated. Molecular dynamics (MD) simulations of galactokinase with the active site residues Arg-37 and Asp-186 altered predicted that two regions (residues 174-179 and 231-240) had different dynamics as a consequence.Interestingly, the same two regions were also affected by alterations in Arg-105, Glu-174 and Arg-228. These three residues were identified as important in catalysis in previous computational studies on human galactokinase. Alteration of Arg-105 to methionine resulted in a modest reduction in activity with little change in stability. When Arg-228 was changed to methionine, the enzyme’s interaction with both ATP and galactose was affected. This variant was significantly less stable than the wild-type protein. Changing Glu-174 to glutamine (but not to aspartate) resulted in no detectable activity and a less stable enzyme. Overall, these combined in silico and in vitro studies demonstrate the importance of a negative charge at position 174 and highlight the critical role of the dynamics in to key regions of the protein. We postulate that these regions may be critical formediating the enzyme’s structure and function.",
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Insight into the mechanism of galactokinase: role of a critical glutamate residue and helix/coil transitions. / McAuley, Margaret; Huang, Meilan; Timson, David.

In: BBA - Proteins and Proteomics, Vol. 1865, No. 3, 24.10.2016, p. 321-328.

Research output: Contribution to journalArticle

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AU - McAuley, Margaret

AU - Huang, Meilan

AU - Timson, David

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