Induction of Human Regulatory T Cells with Bacterial Superantigens

Stefano Caserta, Nadia Terrazzini, Amanda L. Taylor, Martin Llewelyn

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNChapter

Abstract

Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.
Original languageEnglish
Title of host publicationSuperantigens: Methods and protocols
EditorsAmanda J. Brosnahan
Place of PublicationUK
PublisherSpringer
Pages181-206
Number of pages26
ISBN (Electronic)9781493933440
ISBN (Print)9781493933426
DOIs
Publication statusPublished - 17 Dec 2015

Publication series

NameMethods in Molecular Biology

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  • Cite this

    Caserta, S., Terrazzini, N., Taylor, A. L., & Llewelyn, M. (2015). Induction of Human Regulatory T Cells with Bacterial Superantigens. In A. J. Brosnahan (Ed.), Superantigens: Methods and protocols (pp. 181-206). (Methods in Molecular Biology). Springer. https://doi.org/10.1007/978-1-4939-3344-0_16