Induction of Human Regulatory T Cells with Bacterial Superantigens

Stefano Caserta; Nadia Terrazzini; Amanda L. Taylor; Martin Llewelyn

doi:10.1007/978-1-4939-3344-0_16

Induction of Human Regulatory T Cells with Bacterial Superantigens

Stefano Caserta, Nadia Terrazzini, Amanda L. Taylor, Martin Llewelyn

Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Chapter

Abstract

Regulatory T cells (Tregs) that suppress the activation of immune effector cells limit immunopathology and are fast emerging as therapeutic targets for autoimmune and cancer disease. Tools enabling Treg in vitro-induction, expansion, and characterization and manipulation will help future clinical developments. In this chapter, we describe in detail how to use bacterial superantigens to induce human Tregs efficiently from peripheral blood mononuclear cells. How to assess human Treg phenotype and suppressive capacity are also described. Technical details, variations, and alternative experimental conditions are provided.

Original language	English
Title of host publication	Superantigens: Methods and protocols
Editors	Amanda J. Brosnahan
Place of Publication	UK
Publisher	Springer
Pages	181-206
Number of pages	26
ISBN (Electronic)	9781493933440
ISBN (Print)	9781493933426
DOIs	https://doi.org/10.1007/978-1-4939-3344-0_16
Publication status	Published - 17 Dec 2015

Publication series

Name	Methods in Molecular Biology

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10.1007/978-1-4939-3344-0_16Licence: Unspecified

Nadia Terrazzini
- N.Terrazzinibrighton.acuk
- School of Applied Sciences - Principal Lecturer
- Centre for Lifelong Health
- Centre for Regenerative Medicine and Devices
Person: Academic

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Induction of Human Regulatory T Cells with Bacterial Superantigens

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Nadia Terrazzini

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