TY - JOUR
T1 - In vivo absorption, metabolism and urinary excretion of alpha, beta-Unsaturated aldehydes in experimental animals-Relevance to the development of cardiovascular diseases by the dietary ingestion of thermally stressed polyunsaturate-rich culinary oils
AU - Grootveld, Martin C.
AU - Atherton, Martin D.
AU - Sheerin, Angela
AU - Hawkes, Jane
AU - Blake, David R.
AU - Richens, Trevor
AU - Silwood, Christopher J.L.
AU - Lynch, Edward J.
AU - Claxson, Andrew W.D.
PY - 1998/3/15
Y1 - 1998/3/15
N2 - Thermal stressing of polyunsaturated fatty acid (PUFA)-rich culinary oils according to routine frying or cooking practices generates high levels of cytotoxic aldehydic prod- ucts (predominantly trans-2-alkenals, trans,trans-alka-2,4- dienals, cis,trans-alka-2,4-dienals, and n-alkanals), species arising from the fragmentation of conjugated hydroperoxy- diene precursors. In this investigation we demonstrate that typical trans-2-alkenal compounds known to be produced from the thermally induced autoxidation of PUFAs are readily absorbed from the gut into the systemic circulation in vivo, metabolized (primarily via the addition of glu- tathione across their electrophilic carbon-carbon double bonds), and excreted in the urine as C-3 mercapturate con- jugates in rats. Since such aldehydic products are damaging to human health, the results obtained from our investiga- tions indicate that the dietary ingestion of thermally, autox- idatively stressed PUFA-rich culinary oils promotes the in- duction, development, and progression of cardiovascular diseases.
AB - Thermal stressing of polyunsaturated fatty acid (PUFA)-rich culinary oils according to routine frying or cooking practices generates high levels of cytotoxic aldehydic prod- ucts (predominantly trans-2-alkenals, trans,trans-alka-2,4- dienals, cis,trans-alka-2,4-dienals, and n-alkanals), species arising from the fragmentation of conjugated hydroperoxy- diene precursors. In this investigation we demonstrate that typical trans-2-alkenal compounds known to be produced from the thermally induced autoxidation of PUFAs are readily absorbed from the gut into the systemic circulation in vivo, metabolized (primarily via the addition of glu- tathione across their electrophilic carbon-carbon double bonds), and excreted in the urine as C-3 mercapturate con- jugates in rats. Since such aldehydic products are damaging to human health, the results obtained from our investiga- tions indicate that the dietary ingestion of thermally, autox- idatively stressed PUFA-rich culinary oils promotes the in- duction, development, and progression of cardiovascular diseases.
U2 - 10.1172/JCI1314
DO - 10.1172/JCI1314
M3 - Article
VL - 101
SP - 1210
EP - 1218
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
SN - 0021-9738
IS - 6
ER -