In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography

K Madi, KA Staines, BK Bay, B Javaheri, H Geng, AJ Bodey, S Cartmell, AA Pitsillides, PD Lee

Research output: Contribution to journalArticle

Abstract

Imaging techniques for quantifying changes in the hierarchical structure of deforming joints are constrained by destructive sample treatments, sample-size restrictions and lengthy scan times. Here, we report the use of fast low-dose pink-beam synchrotron X-ray tomography in combination with mechanical loading at nanometric precision for in situ imaging, at resolutions below 100 nm, of the mechanical strain in intact untreated joints under physiologically realistic conditions. We show that in young, older and osteoarthritic mice, hierarchical changes in tissue structure and mechanical behaviour can be simultaneously visualized, and that the tissue structure at the cellular level correlates with the mechanical performance of the whole joint. We also use the tomographic approach to study the colocalization of tissue strains to specific chondrocyte lacunar organizations within intact loaded joints and to explore the role of calcified-cartilage stiffness on the biomechanics of healthy and pathological joints.

Original languageEnglish
JournalNature biomedical engineering
DOIs
Publication statusPublished - 25 Nov 2019

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X Ray Tomography
Synchrotrons
Tomography
Joints
Tissue
X rays
Imaging techniques
Biomechanics
Cartilage
Dosimetry
Stiffness
Cellular Structures
Chondrocytes
Biomechanical Phenomena
Sample Size

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Madi, K., Staines, KA., Bay, BK., Javaheri, B., Geng, H., Bodey, AJ., ... Lee, PD. (2019). In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography. Nature biomedical engineering. https://doi.org/10.1038/s41551-019-0477-1
Madi, K ; Staines, KA ; Bay, BK ; Javaheri, B ; Geng, H ; Bodey, AJ ; Cartmell, S ; Pitsillides, AA ; Lee, PD. / In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography. In: Nature biomedical engineering. 2019.
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Madi, K, Staines, KA, Bay, BK, Javaheri, B, Geng, H, Bodey, AJ, Cartmell, S, Pitsillides, AA & Lee, PD 2019, 'In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography', Nature biomedical engineering. https://doi.org/10.1038/s41551-019-0477-1

In situ characterization of nanoscale strains in loaded whole joints via synchrotron X-ray tomography. / Madi, K; Staines, KA; Bay, BK; Javaheri, B; Geng, H; Bodey, AJ; Cartmell, S; Pitsillides, AA; Lee, PD.

In: Nature biomedical engineering, 25.11.2019.

Research output: Contribution to journalArticle

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