IL-7 unveils pathogen-specific T cells by enhancing antigen-recall responses

Nadia Terrazzini, Paola Mantegani, Florian Kern, Claudio Fortis, Anna Mondino, Stefano Caserta

Research output: Contribution to journalArticle

Abstract

BACKGROUND: IL-7 promotes the generation, expansion and survival of memory T cells. Previous mouse and human studies showed that IL-7 can support immune cell reconstitution in lymphopenic conditions, expand tumor-reactive T cells for adoptive immunotherapy and enhance effector cytokine expression by autoreactive T cells. Whether pathogen-reactive T cells also benefit from IL-7 exposure remains unknown. METHODS: Here we investigated this issue in cultures of peripheral blood mononuclear cells (PBMCs) derived from patients infected with various endemic pathogens. After short-term exposure to IL-7, we measured PBMC responses to antigens (Ag) derived from pathogens, such as Mycobacterium tuberculosis (MTB), Candida albicans (Ca) and Cytomegalovirus (CMV), and to the superantigen Staphylococcus aureus enterotoxin B (SEB). RESULTS: We found that IL-7 favoured the expansion and, in some instances, the uncovering of pathogen-reactive CD4 T cells, by promoting pathogen-specific IFNɣ, IL-2 and TNF recall responses. CONCLUSIONS: Our findings indicate that IL-7 unveils and supports re-activation of pathogen-specific T cells with possible diagnostic, prognostic and therapeutic significance, of clinical value especially in conditions of pathogen persistence and chronic infection.
Original languageEnglish
Pages (from-to)1997–2007
JournalJournal of Infectious Diseases
Volume217
Issue number12
DOIs
Publication statusPublished - 28 Feb 2018

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Interleukin-7
T-Lymphocytes
Antigens
Blood Cells
Adoptive Immunotherapy
Superantigens
Candida albicans
Cytomegalovirus
Mycobacterium tuberculosis
Interleukin-2
Staphylococcus aureus
Cytokines
Survival
Infection
Neoplasms

Bibliographical note

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Cite this

Terrazzini, Nadia ; Mantegani, Paola ; Kern, Florian ; Fortis, Claudio ; Mondino, Anna ; Caserta, Stefano. / IL-7 unveils pathogen-specific T cells by enhancing antigen-recall responses. In: Journal of Infectious Diseases. 2018 ; Vol. 217, No. 12. pp. 1997–2007.
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abstract = "BACKGROUND: IL-7 promotes the generation, expansion and survival of memory T cells. Previous mouse and human studies showed that IL-7 can support immune cell reconstitution in lymphopenic conditions, expand tumor-reactive T cells for adoptive immunotherapy and enhance effector cytokine expression by autoreactive T cells. Whether pathogen-reactive T cells also benefit from IL-7 exposure remains unknown. METHODS: Here we investigated this issue in cultures of peripheral blood mononuclear cells (PBMCs) derived from patients infected with various endemic pathogens. After short-term exposure to IL-7, we measured PBMC responses to antigens (Ag) derived from pathogens, such as Mycobacterium tuberculosis (MTB), Candida albicans (Ca) and Cytomegalovirus (CMV), and to the superantigen Staphylococcus aureus enterotoxin B (SEB). RESULTS: We found that IL-7 favoured the expansion and, in some instances, the uncovering of pathogen-reactive CD4 T cells, by promoting pathogen-specific IFNɣ, IL-2 and TNF recall responses. CONCLUSIONS: Our findings indicate that IL-7 unveils and supports re-activation of pathogen-specific T cells with possible diagnostic, prognostic and therapeutic significance, of clinical value especially in conditions of pathogen persistence and chronic infection.",
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Terrazzini, N, Mantegani, P, Kern, F, Fortis, C, Mondino, A & Caserta, S 2018, 'IL-7 unveils pathogen-specific T cells by enhancing antigen-recall responses', Journal of Infectious Diseases, vol. 217, no. 12, pp. 1997–2007. https://doi.org/10.1093/infdis/jiy096

IL-7 unveils pathogen-specific T cells by enhancing antigen-recall responses. / Terrazzini, Nadia; Mantegani, Paola; Kern, Florian; Fortis, Claudio; Mondino, Anna; Caserta, Stefano.

In: Journal of Infectious Diseases, Vol. 217, No. 12, 28.02.2018, p. 1997–2007.

Research output: Contribution to journalArticle

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AU - Terrazzini, Nadia

AU - Mantegani, Paola

AU - Kern, Florian

AU - Fortis, Claudio

AU - Mondino, Anna

AU - Caserta, Stefano

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N2 - BACKGROUND: IL-7 promotes the generation, expansion and survival of memory T cells. Previous mouse and human studies showed that IL-7 can support immune cell reconstitution in lymphopenic conditions, expand tumor-reactive T cells for adoptive immunotherapy and enhance effector cytokine expression by autoreactive T cells. Whether pathogen-reactive T cells also benefit from IL-7 exposure remains unknown. METHODS: Here we investigated this issue in cultures of peripheral blood mononuclear cells (PBMCs) derived from patients infected with various endemic pathogens. After short-term exposure to IL-7, we measured PBMC responses to antigens (Ag) derived from pathogens, such as Mycobacterium tuberculosis (MTB), Candida albicans (Ca) and Cytomegalovirus (CMV), and to the superantigen Staphylococcus aureus enterotoxin B (SEB). RESULTS: We found that IL-7 favoured the expansion and, in some instances, the uncovering of pathogen-reactive CD4 T cells, by promoting pathogen-specific IFNɣ, IL-2 and TNF recall responses. CONCLUSIONS: Our findings indicate that IL-7 unveils and supports re-activation of pathogen-specific T cells with possible diagnostic, prognostic and therapeutic significance, of clinical value especially in conditions of pathogen persistence and chronic infection.

AB - BACKGROUND: IL-7 promotes the generation, expansion and survival of memory T cells. Previous mouse and human studies showed that IL-7 can support immune cell reconstitution in lymphopenic conditions, expand tumor-reactive T cells for adoptive immunotherapy and enhance effector cytokine expression by autoreactive T cells. Whether pathogen-reactive T cells also benefit from IL-7 exposure remains unknown. METHODS: Here we investigated this issue in cultures of peripheral blood mononuclear cells (PBMCs) derived from patients infected with various endemic pathogens. After short-term exposure to IL-7, we measured PBMC responses to antigens (Ag) derived from pathogens, such as Mycobacterium tuberculosis (MTB), Candida albicans (Ca) and Cytomegalovirus (CMV), and to the superantigen Staphylococcus aureus enterotoxin B (SEB). RESULTS: We found that IL-7 favoured the expansion and, in some instances, the uncovering of pathogen-reactive CD4 T cells, by promoting pathogen-specific IFNɣ, IL-2 and TNF recall responses. CONCLUSIONS: Our findings indicate that IL-7 unveils and supports re-activation of pathogen-specific T cells with possible diagnostic, prognostic and therapeutic significance, of clinical value especially in conditions of pathogen persistence and chronic infection.

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