Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1: 1 stoichiometry

David J. Timson; Helen C. Ross; Richard J. Reece

doi:10.1042/0264-6021:3630515

Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1: 1 stoichiometry

David J. Timson, Helen C. Ross, Richard J. Reece

Research output: Contribution to journal › Article › peer-review

Abstract

The genes encoding the enzymes required for galactose metabolism in Saccharomyces cerevisiae are controlled at the level of transcription by a genetic switch consisting of three proteins: a transcriptional activator, Gal4p; a transcriptional repressor, Gal80p; and a ligand sensor, Gal3p. The switch is turned on in the presence of two small molecule ligands, galactose and ATP. Gal3p shows a high degree of sequence identity with Gal1p, the yeast galactokinase. We have mapped the interaction between Gal80p and Gal3p, which only occurs in the presence of both ligands, using protease protection experiments and have shown that this involves amino acid residue 331 of Gal80p. Gel-filtration experiments indicate that Gal3p, or the galactokinase Gal1p, interact directly with Gal80p to form a complex with 1:1 stoichiometry.

Original language	English
Pages (from-to)	515-520
Number of pages	6
Journal	Biochemical Journal
Volume	363
Issue number	3
DOIs	https://doi.org/10.1042/0264-6021:3630515
Publication status	Published - 1 May 2002

Keywords

Gal4p
Galactose
Gene expression
Protein-protein interaction
Yeast

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10.1042/0264-6021:3630515

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title = "Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1: 1 stoichiometry",

abstract = "The genes encoding the enzymes required for galactose metabolism in Saccharomyces cerevisiae are controlled at the level of transcription by a genetic switch consisting of three proteins: a transcriptional activator, Gal4p; a transcriptional repressor, Gal80p; and a ligand sensor, Gal3p. The switch is turned on in the presence of two small molecule ligands, galactose and ATP. Gal3p shows a high degree of sequence identity with Gal1p, the yeast galactokinase. We have mapped the interaction between Gal80p and Gal3p, which only occurs in the presence of both ligands, using protease protection experiments and have shown that this involves amino acid residue 331 of Gal80p. Gel-filtration experiments indicate that Gal3p, or the galactokinase Gal1p, interact directly with Gal80p to form a complex with 1:1 stoichiometry.",

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T1 - Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1

T2 - 1 stoichiometry

AU - Timson, David J.

AU - Ross, Helen C.

AU - Reece, Richard J.

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N2 - The genes encoding the enzymes required for galactose metabolism in Saccharomyces cerevisiae are controlled at the level of transcription by a genetic switch consisting of three proteins: a transcriptional activator, Gal4p; a transcriptional repressor, Gal80p; and a ligand sensor, Gal3p. The switch is turned on in the presence of two small molecule ligands, galactose and ATP. Gal3p shows a high degree of sequence identity with Gal1p, the yeast galactokinase. We have mapped the interaction between Gal80p and Gal3p, which only occurs in the presence of both ligands, using protease protection experiments and have shown that this involves amino acid residue 331 of Gal80p. Gel-filtration experiments indicate that Gal3p, or the galactokinase Gal1p, interact directly with Gal80p to form a complex with 1:1 stoichiometry.

AB - The genes encoding the enzymes required for galactose metabolism in Saccharomyces cerevisiae are controlled at the level of transcription by a genetic switch consisting of three proteins: a transcriptional activator, Gal4p; a transcriptional repressor, Gal80p; and a ligand sensor, Gal3p. The switch is turned on in the presence of two small molecule ligands, galactose and ATP. Gal3p shows a high degree of sequence identity with Gal1p, the yeast galactokinase. We have mapped the interaction between Gal80p and Gal3p, which only occurs in the presence of both ligands, using protease protection experiments and have shown that this involves amino acid residue 331 of Gal80p. Gel-filtration experiments indicate that Gal3p, or the galactokinase Gal1p, interact directly with Gal80p to form a complex with 1:1 stoichiometry.

KW - Gal4p

KW - Galactose

KW - Gene expression

KW - Protein-protein interaction

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JO - Biochemical Journal

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Gal3p and Gal1p interact with the transcriptional repressor Gal80p to form a complex of 1: 1 stoichiometry

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