New refrigerants are required for cooling systems due to the fact that refrigerants like R134a are about to be phased out. This paper presents a comparison between the flow boiling heat transfer and pressure drop results of refrigerants R245fa and R134a. The experiments with R245fa were conducted in a vertical cold drawn stainless steel tube with an inner diameter of 1.1 mm and heated length of 150 mm. Experimental conditions include: mass flux range 100–400 kg/m2s, heat flux range 10–60 kW/m2, pressures of 8 and 10 bar and 1.9 and 2.5 bar for R134a and R245fa corresponding to saturated temperatures 31 °C and 39 °C and exit vapour quality range 0–0.95. The data for R134a were obtained earlier using the same experimental facility at the same experimental conditions and with the same test tube. The results demonstrated that refrigerant properties have a significant effect on heat transfer and pressure drop. The pressure drop of R245fa is higher by up to 300% compared to that of R134a at similar conditions. In addition, the effect of mass flux and heat flux on the local flow boiling heat transfer coefficient was different. Heat transfer coefficients of R245fa showed a greater dependence on vapour quality. The agreement with past heat transfer correlations is better with R134a than with R245fa.