Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis)

E. Cubero-Leon, Corina Ciocan, E.M. Hill, M. Osada, M. Kishida, N. Itoh, R. Kondo, C. Minier, J.M. Rotchell

Research output: Contribution to journalArticle

Abstract

Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in the reproductive process and is subjected to hormonal control by serotonin (5-HT), prostaglandins (synthesised by cyclooxygenases-COX) and steroids such as 17beta-estradiol (E2). Here, we examine the responses of 5-HT receptor and COX mRNA expression in mussels, Mytilus edulis, exposed to estrogenic compounds during different stages of their reproductive cycle. In mature mussels, 5-HT receptor mRNA expression decreased following E2 exposure. The opposite trend was observed in mussels at early gametogenesis stages. COX mRNA expression levels at both stages were generally decreased by E2 exposure. Mussels at early gametogenesis stages were also exposed to ethynylestradiol (EE2) and estradiol benzoate (EB) and a significant increase in 5-HT receptor mRNA expression was observed with both xeno-estrogens. COX expression levels were increased with EB exposure but no significant effects were found with EE2 exposure. These results show that the natural estrogen, E2, as well as the synthetic estrogen, EE2, induce alterations, dependent on reproductive stage, in the mRNA expression levels of 5-HT receptor and/or COX in the marine bivalve M. edulis
Original languageEnglish
Pages (from-to)178-187
Number of pages10
JournalAquatic Toxicology
Volume98
Issue number2
Publication statusPublished - 10 Jun 2010

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prostaglandin synthase
Mytilus edulis
mussels
gonads
gametogenesis
estradiol
benzoates
serotonin
estrogens
Bivalvia
synthetic estrogens
feminization
hormonal regulation
aquatic environment
prostaglandins
steroids
invertebrates
estrogen receptors
serotonin receptors
fish

Keywords

  • Mytilus edulis
  • Cyclooxygenase
  • Serotonin receptor
  • 17 beta-estradiol
  • Ethynylestradiol
  • Estradiol benzoate
  • Endocrine disruption

Cite this

Cubero-Leon, E., Ciocan, C., Hill, E. M., Osada, M., Kishida, M., Itoh, N., ... Rotchell, J. M. (2010). Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). Aquatic Toxicology, 98(2), 178-187.
Cubero-Leon, E. ; Ciocan, Corina ; Hill, E.M. ; Osada, M. ; Kishida, M. ; Itoh, N. ; Kondo, R. ; Minier, C. ; Rotchell, J.M. / Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). In: Aquatic Toxicology. 2010 ; Vol. 98, No. 2. pp. 178-187.
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Cubero-Leon, E, Ciocan, C, Hill, EM, Osada, M, Kishida, M, Itoh, N, Kondo, R, Minier, C & Rotchell, JM 2010, 'Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis)', Aquatic Toxicology, vol. 98, no. 2, pp. 178-187.

Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis). / Cubero-Leon, E.; Ciocan, Corina; Hill, E.M.; Osada, M.; Kishida, M.; Itoh, N.; Kondo, R.; Minier, C.; Rotchell, J.M.

In: Aquatic Toxicology, Vol. 98, No. 2, 10.06.2010, p. 178-187.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Estrogens disrupt serotonin receptor and cyclooxygenase mRNA expression in the gonads of mussels (Mytilus edulis)

AU - Cubero-Leon, E.

AU - Ciocan, Corina

AU - Hill, E.M.

AU - Osada, M.

AU - Kishida, M.

AU - Itoh, N.

AU - Kondo, R.

AU - Minier, C.

AU - Rotchell, J.M.

PY - 2010/6/10

Y1 - 2010/6/10

N2 - Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in the reproductive process and is subjected to hormonal control by serotonin (5-HT), prostaglandins (synthesised by cyclooxygenases-COX) and steroids such as 17beta-estradiol (E2). Here, we examine the responses of 5-HT receptor and COX mRNA expression in mussels, Mytilus edulis, exposed to estrogenic compounds during different stages of their reproductive cycle. In mature mussels, 5-HT receptor mRNA expression decreased following E2 exposure. The opposite trend was observed in mussels at early gametogenesis stages. COX mRNA expression levels at both stages were generally decreased by E2 exposure. Mussels at early gametogenesis stages were also exposed to ethynylestradiol (EE2) and estradiol benzoate (EB) and a significant increase in 5-HT receptor mRNA expression was observed with both xeno-estrogens. COX expression levels were increased with EB exposure but no significant effects were found with EE2 exposure. These results show that the natural estrogen, E2, as well as the synthetic estrogen, EE2, induce alterations, dependent on reproductive stage, in the mRNA expression levels of 5-HT receptor and/or COX in the marine bivalve M. edulis

AB - Estrogenic contaminants in the aquatic environment are associated with feminisation of male fish, however their effects on some invertebrate species, such as bivalve molluscs, have yet to be characterised. Gametogenesis represents a critical step in the reproductive process and is subjected to hormonal control by serotonin (5-HT), prostaglandins (synthesised by cyclooxygenases-COX) and steroids such as 17beta-estradiol (E2). Here, we examine the responses of 5-HT receptor and COX mRNA expression in mussels, Mytilus edulis, exposed to estrogenic compounds during different stages of their reproductive cycle. In mature mussels, 5-HT receptor mRNA expression decreased following E2 exposure. The opposite trend was observed in mussels at early gametogenesis stages. COX mRNA expression levels at both stages were generally decreased by E2 exposure. Mussels at early gametogenesis stages were also exposed to ethynylestradiol (EE2) and estradiol benzoate (EB) and a significant increase in 5-HT receptor mRNA expression was observed with both xeno-estrogens. COX expression levels were increased with EB exposure but no significant effects were found with EE2 exposure. These results show that the natural estrogen, E2, as well as the synthetic estrogen, EE2, induce alterations, dependent on reproductive stage, in the mRNA expression levels of 5-HT receptor and/or COX in the marine bivalve M. edulis

KW - Mytilus edulis

KW - Cyclooxygenase

KW - Serotonin receptor

KW - 17 beta-estradiol

KW - Ethynylestradiol

KW - Estradiol benzoate

KW - Endocrine disruption

M3 - Article

VL - 98

SP - 178

EP - 187

JO - Aquatic Toxicology

JF - Aquatic Toxicology

SN - 0166-445X

IS - 2

ER -