Estimating stored curvature elastic energy in biological membranes in vitro and in vivo

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNChapterpeer-review

Abstract

Correlations between stored curvature elastic energy (SCE) in biological membranes and enzymatic activity, protein folding and the mechanism by which cells modulate lipid homeostasis have been demonstrated in the literature. Disentangling such relationships from the other physical properties of biological membranes, which might also play a role in regulating enzymatic activity, protein folding and lipid homeostasis, requires reliable methods for measuring SCE in biological membranes to be developed. To date there are no methods that enable SCE to be measured directly in a membrane and therefore it is commonplace for authors to estimate the effects of lipid compositional change on SCE. These approaches tend to be qualitative and based on ‘rule of thumb’ relationships between lipid structure and lipid curvature, determined from lyotropic liquid crystal phase behaviour, or semi-quantitative, utilising measurements of lipid monolayer spontaneous curvatures to estimate the magnitude of curvature elastic stress in a membrane of differing lipid compositions. In this chapter different approaches to estimating SCE within lipid membranes, both in vivo and in vitro, are presented with the aim of enabling researchers to utilise these calculations in their own studies.

Original languageEnglish
Title of host publicationAdvances in Biomembranes and Lipid Self-Assembly
PublisherElsevier
DOIs
Publication statusAccepted/In press - 11 May 2024

Publication series

NameAdvances in Biomembranes and Lipid Self-Assembly
ISSN (Print)2451-9634

Keywords

  • Curvature elastic stress
  • Intrinsic curvature hypothesis
  • Lipid intrinsic curvature
  • Lipid monolayer spontaneous curvature
  • Phospholipid homeostasis

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