Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array

Raphaël Trouillon; Christine Cheung; Bhavik A. Patel; Dong Ku Kang; Soo Ik Chang; Danny O'Hare

Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array

Raphaël Trouillon, Christine Cheung, Bhavik A. Patel, Dong Ku Kang, Soo Ik Chang, Danny O'Hare

Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Conference contribution with ISSN or ISBN › peer-review

Abstract

Angiogenesis, eg. formation of new blood vessels, is regulated by different angiogenic factors. Nitric oxide (NO) is also a very well known biological mediator involved in vascular physiology. This study focuses on relationships between the effect of angiogenin, a major angiogenic factor, and extracellular NO release. NO concentration was sensed electrochemically using a fibronectin coated multiple microelectrode array. Angiogenin was shown to increase NO levels, thus triggering nitric oxide synthase (NOS) activity. Our results also demonstrate that angiogenin activates NOS by interacting with the cell nucleus and via Akt Kinase transduction.

Original language	English
Title of host publication	Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	463-465
Number of pages	3
ISBN (Print)	9780979806421
Publication status	Published - 1 Jan 2009
Event	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: 1 Nov 2009 → 5 Nov 2009

Conference

Conference	13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/Territory	Korea, Republic of
City	Jeju
Period	1/11/09 → 5/11/09

Keywords

Angiogenesis
Angiogenin
Biomeasurements
Electrochemical sensing
Nitric oxide synthase

Cite this

Trouillon, R., Cheung, C., Patel, B. A., Kang, D. K., Chang, S. I., & O'Hare, D. (2009). Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 463-465). Chemical and Biological Microsystems Society.

Trouillon, Raphaël ; Cheung, Christine ; Patel, Bhavik A. et al. / Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 463-465

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abstract = "Angiogenesis, eg. formation of new blood vessels, is regulated by different angiogenic factors. Nitric oxide (NO) is also a very well known biological mediator involved in vascular physiology. This study focuses on relationships between the effect of angiogenin, a major angiogenic factor, and extracellular NO release. NO concentration was sensed electrochemically using a fibronectin coated multiple microelectrode array. Angiogenin was shown to increase NO levels, thus triggering nitric oxide synthase (NOS) activity. Our results also demonstrate that angiogenin activates NOS by interacting with the cell nucleus and via Akt Kinase transduction.",

keywords = "Angiogenesis, Angiogenin, Biomeasurements, Electrochemical sensing, Nitric oxide synthase",

author = "Rapha{\"e}l Trouillon and Christine Cheung and Patel, {Bhavik A.} and Kang, {Dong Ku} and Chang, {Soo Ik} and Danny O'Hare",

year = "2009",

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Trouillon, R, Cheung, C, Patel, BA, Kang, DK, Chang, SI & O'Hare, D 2009, Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 463-465, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 1/11/09.

Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array. / Trouillon, Raphaël; Cheung, Christine; Patel, Bhavik A. et al.
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 463-465.

Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Conference contribution with ISSN or ISBN › peer-review

TY - GEN

T1 - Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array

AU - Trouillon, Raphaël

AU - Cheung, Christine

AU - Patel, Bhavik A.

AU - Kang, Dong Ku

AU - Chang, Soo Ik

AU - O'Hare, Danny

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Angiogenesis, eg. formation of new blood vessels, is regulated by different angiogenic factors. Nitric oxide (NO) is also a very well known biological mediator involved in vascular physiology. This study focuses on relationships between the effect of angiogenin, a major angiogenic factor, and extracellular NO release. NO concentration was sensed electrochemically using a fibronectin coated multiple microelectrode array. Angiogenin was shown to increase NO levels, thus triggering nitric oxide synthase (NOS) activity. Our results also demonstrate that angiogenin activates NOS by interacting with the cell nucleus and via Akt Kinase transduction.

AB - Angiogenesis, eg. formation of new blood vessels, is regulated by different angiogenic factors. Nitric oxide (NO) is also a very well known biological mediator involved in vascular physiology. This study focuses on relationships between the effect of angiogenin, a major angiogenic factor, and extracellular NO release. NO concentration was sensed electrochemically using a fibronectin coated multiple microelectrode array. Angiogenin was shown to increase NO levels, thus triggering nitric oxide synthase (NOS) activity. Our results also demonstrate that angiogenin activates NOS by interacting with the cell nucleus and via Akt Kinase transduction.

KW - Angiogenesis

KW - Angiogenin

KW - Biomeasurements

KW - Electrochemical sensing

KW - Nitric oxide synthase

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M3 - Conference contribution with ISSN or ISBN

AN - SCOPUS:84901762001

SN - 9780979806421

SP - 463

EP - 465

BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009

Y2 - 1 November 2009 through 5 November 2009

ER -

Trouillon R, Cheung C, Patel BA, Kang DK, Chang SI, O'Hare D. Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 463-465

Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array

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