Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array

Raphaël Trouillon, Christine Cheung, Bhavik A. Patel, Dong Ku Kang, Soo Ik Chang, Danny O'Hare

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNConference contribution with ISSN or ISBN

Abstract

Angiogenesis, eg. formation of new blood vessels, is regulated by different angiogenic factors. Nitric oxide (NO) is also a very well known biological mediator involved in vascular physiology. This study focuses on relationships between the effect of angiogenin, a major angiogenic factor, and extracellular NO release. NO concentration was sensed electrochemically using a fibronectin coated multiple microelectrode array. Angiogenin was shown to increase NO levels, thus triggering nitric oxide synthase (NOS) activity. Our results also demonstrate that angiogenin activates NOS by interacting with the cell nucleus and via Akt Kinase transduction.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages463-465
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 1 Jan 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Angiogenesis
  • Angiogenin
  • Biomeasurements
  • Electrochemical sensing
  • Nitric oxide synthase

Fingerprint Dive into the research topics of 'Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array'. Together they form a unique fingerprint.

  • Cite this

    Trouillon, R., Cheung, C., Patel, B. A., Kang, D. K., Chang, S. I., & O'Hare, D. (2009). Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 463-465). Chemical and Biological Microsystems Society.