Electrochemical sensing of the effect of angiogenic factors on nitric oxide release in endothelial cells using a biocompatible microelectrode array

Raphaël Trouillon, Christine Cheung, Bhavik A. Patel, Dong Ku Kang, Soo Ik Chang, Danny O'Hare

    Research output: Chapter in Book/Conference proceeding with ISSN or ISBNConference contribution with ISSN or ISBNpeer-review

    Abstract

    Angiogenesis, eg. formation of new blood vessels, is regulated by different angiogenic factors. Nitric oxide (NO) is also a very well known biological mediator involved in vascular physiology. This study focuses on relationships between the effect of angiogenin, a major angiogenic factor, and extracellular NO release. NO concentration was sensed electrochemically using a fibronectin coated multiple microelectrode array. Angiogenin was shown to increase NO levels, thus triggering nitric oxide synthase (NOS) activity. Our results also demonstrate that angiogenin activates NOS by interacting with the cell nucleus and via Akt Kinase transduction.

    Original languageEnglish
    Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Pages463-465
    Number of pages3
    ISBN (Print)9780979806421
    Publication statusPublished - 1 Jan 2009
    Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
    Duration: 1 Nov 20095 Nov 2009

    Conference

    Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
    Country/TerritoryKorea, Republic of
    CityJeju
    Period1/11/095/11/09

    Keywords

    • Angiogenesis
    • Angiogenin
    • Biomeasurements
    • Electrochemical sensing
    • Nitric oxide synthase

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