TY - JOUR
T1 - Effects of short-term exposure of paracetamol in the gonads of blue mussels Mytilus edulis
AU - Koagouw, Wulan
AU - Ciocan, Corina
PY - 2019/11/20
Y1 - 2019/11/20
N2 - A growing body of literature suggests that pharmaceutical contamination poses an increasing risk to marine ecosystems. Paracetamol or acetaminophen is the most widely used medicine in the world and has recently been detected in seawater. Here, we present the results of 7 days’ exposure of blue mussel adults to 40 ng/L, 250 ng/L and 100 μg/L of paracetamol. Histopathology shows that haemocytic infiltration is the most observed condition in the exposed mussels. The mRNA expression of VTG, V9, ER2, HSP70, CASP8, BCL2 and FAS in mussel gonads present different patterns of downregulation. VTG and CASP8 mRNA expression show downregulation in all exposed mussels, irrespective of sex. The V9, HSP70, BCL2 and FAS transcripts follow a concentration-dependent variation in gene expression and may therefore be considered good biomarker candidates. ER2 mRNA expression shows a downregulated trend, with a clearer dose-response relationship in males. In conclusion, this study suggests that paracetamol has the potential to alter the expression of several genes related to processes occurring in the reproductive system and may therefore impair reproduction in blue mussels
AB - A growing body of literature suggests that pharmaceutical contamination poses an increasing risk to marine ecosystems. Paracetamol or acetaminophen is the most widely used medicine in the world and has recently been detected in seawater. Here, we present the results of 7 days’ exposure of blue mussel adults to 40 ng/L, 250 ng/L and 100 μg/L of paracetamol. Histopathology shows that haemocytic infiltration is the most observed condition in the exposed mussels. The mRNA expression of VTG, V9, ER2, HSP70, CASP8, BCL2 and FAS in mussel gonads present different patterns of downregulation. VTG and CASP8 mRNA expression show downregulation in all exposed mussels, irrespective of sex. The V9, HSP70, BCL2 and FAS transcripts follow a concentration-dependent variation in gene expression and may therefore be considered good biomarker candidates. ER2 mRNA expression shows a downregulated trend, with a clearer dose-response relationship in males. In conclusion, this study suggests that paracetamol has the potential to alter the expression of several genes related to processes occurring in the reproductive system and may therefore impair reproduction in blue mussels
KW - Short-term exposure
KW - Paracetamol
KW - Blue mussels
KW - Gene expression
KW - Histology
KW - Seawater
KW - Pharmaceuticals
KW - Marine pollution
UR - http://www.scopus.com/inward/record.url?scp=85075361923&partnerID=8YFLogxK
U2 - 10.1007/s11356-019-06861-w
DO - 10.1007/s11356-019-06861-w
M3 - Article
SN - 0944-1344
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
ER -