Effective microorganism – X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions

Lee Taylor; Ben J. Lee; Oliver Gibson; Adrian W. Midgley; Peter Watt; A.R. Mauger; Paul C. Castle

doi:10.1080/15438627.2015.1126279

Effective microorganism – X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions

Lee Taylor, Ben J. Lee, Oliver Gibson, Adrian W. Midgley, Peter Watt, A.R. Mauger, Paul C. Castle

Centre for Regenerative Medicine and Devices

Research output: Contribution to journal › Article › peer-review

Abstract

This study determined the effectiveness of antioxidant supplementa- tion on high-intensity exercise-heat stress. Six males completed a high- intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following 7 days supplementation with 70 ml·day −1 effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml−1) compared to TEMP (+0.8 ng·ml−1). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml−1) versus TEMP (+0.10 U·ml−1) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.

Original language	English
Pages (from-to)	130-144
Number of pages	15
Journal	Research in Sports Medicine
Volume	24
Issue number	2
DOIs	https://doi.org/10.1080/15438627.2015.1126279
Publication status	Published - 31 Mar 2016

Bibliographical note

This is an Accepted Manuscript of an article published by Taylor & Francis in Research in Sports Medicine on 31/03/2016, available online: http://www.tandfonline.com/10.1080/15438627.2015.1126279

Keywords

heat shock proteins (HSP)
oxidative stress
redox balance
repeated sprint
exercise
HIT
human

Access to Document

10.1080/15438627.2015.1126279Licence: Unspecified

Effective microorganism X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot humid conditions.pdfAccepted author manuscript, 443 KBLicence: Unspecified

http://www.tandfonline.com/doi/full/10.1080/15438627.2015.1126279Licence: Unspecified

Cite this

@article{1a6681fade4144249956ece6e864cbc7,

title = "Effective microorganism – X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions",

abstract = "This study determined the effectiveness of antioxidant supplementa- tion on high-intensity exercise-heat stress. Six males completed a high- intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following 7 days supplementation with 70 ml·day −1 effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml−1) compared to TEMP (+0.8 ng·ml−1). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml−1) versus TEMP (+0.10 U·ml−1) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.",

keywords = "heat shock proteins (HSP), oxidative stress, redox balance, repeated sprint, exercise, HIT, human",

author = "Lee Taylor and Lee, {Ben J.} and Oliver Gibson and Midgley, {Adrian W.} and Peter Watt and A.R. Mauger and Castle, {Paul C.}",

note = "This is an Accepted Manuscript of an article published by Taylor & Francis in Research in Sports Medicine on 31/03/2016, available online: http://www.tandfonline.com/10.1080/15438627.2015.1126279",

year = "2016",

month = mar,

day = "31",

doi = "10.1080/15438627.2015.1126279",

language = "English",

volume = "24",

pages = "130--144",

journal = "Research in Sports Medicine",

issn = "1543-8627",

number = "2",

}

TY - JOUR

T1 - Effective microorganism – X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions

AU - Taylor, Lee

AU - Lee, Ben J.

AU - Gibson, Oliver

AU - Midgley, Adrian W.

AU - Watt, Peter

AU - Mauger, A.R.

AU - Castle, Paul C.

N1 - This is an Accepted Manuscript of an article published by Taylor & Francis in Research in Sports Medicine on 31/03/2016, available online: http://www.tandfonline.com/10.1080/15438627.2015.1126279

PY - 2016/3/31

Y1 - 2016/3/31

N2 - This study determined the effectiveness of antioxidant supplementa- tion on high-intensity exercise-heat stress. Six males completed a high- intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following 7 days supplementation with 70 ml·day −1 effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml−1) compared to TEMP (+0.8 ng·ml−1). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml−1) versus TEMP (+0.10 U·ml−1) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.

AB - This study determined the effectiveness of antioxidant supplementa- tion on high-intensity exercise-heat stress. Six males completed a high- intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following 7 days supplementation with 70 ml·day −1 effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml−1) compared to TEMP (+0.8 ng·ml−1). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml−1) versus TEMP (+0.10 U·ml−1) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.

KW - heat shock proteins (HSP)

KW - oxidative stress

KW - redox balance

KW - repeated sprint

KW - exercise

KW - HIT

KW - human

U2 - 10.1080/15438627.2015.1126279

DO - 10.1080/15438627.2015.1126279

M3 - Article

SN - 1543-8627

VL - 24

SP - 130

EP - 144

JO - Research in Sports Medicine

JF - Research in Sports Medicine

IS - 2

ER -

Effective microorganism – X attenuates circulating superoxide dismutase following an acute bout of intermittent running in hot, humid conditions

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this