This study determined the effectiveness of antioxidant supplementa- tion on high-intensity exercise-heat stress. Six males completed a high- intensity running protocol twice in temperate conditions (TEMP; 20.4°C), and twice in hot conditions (HOT; 34.7°C). Trials were completed following 7 days supplementation with 70 ml·day −1 effective microorganism-X (EM-X; TEMPEMX or HOTEMX) or placebo (TEMPPLA or HOTPLA). Plasma extracellular Hsp72 (eHsp72) and superoxide dismutase (SOD) were measured by ELISA. eHsp72 and SOD increased pre-post exercise (p < 0.001), with greater eHsp72 (p < 0.001) increases observed in HOT (+1.5 ng·ml−1) compared to TEMP (+0.8 ng·ml−1). EM-X did not influence eHsp72 (p > 0.05). Greater (p < 0.001) SOD increases were observed in HOT (+0.22 U·ml−1) versus TEMP (+0.10 U·ml−1) with SOD reduced in HOTEMX versus HOTPLA (p = 0.001). Physiological and perceptual responses were all greater (p < 0.001) in HOT versus TEMP conditions, with no difference followed EM-X (p > 0.05). EM-X supplementation attenuated the SOD increases following HOT, potentiating its application as an ergogenic aid to ameliorate oxidative stress.
Bibliographical noteThis is an Accepted Manuscript of an article published by Taylor & Francis in Research in Sports Medicine on 31/03/2016, available online: http://www.tandfonline.com/10.1080/15438627.2015.1126279
- heat shock proteins (HSP)
- oxidative stress
- redox balance
- repeated sprint