Dietary hydroxycinnamates and resveratrol reduce oxidant injury in renal cells

Prabal Chatterjee, Benjamin Elisha-Lambert, Ananth Pannala

Research output: Contribution to conferenceAbstract


Methods: An in vitro model of nephrotoxic AKI was developed using paraquat (PQ) to generate OS and oxidant injury in the NRK-52E rat renal proximal tubular cell-line. Both time (3mM, over 24 hours) and concentration (0-5mM) dependent profiles of protection by hydroxycinnamates and resveratrol against PQ-mediated oxidant injury were determined. NRK-52E cells were co-incubated with 3mM PQ and increasing concentrations of hydroxycinnamates or resveratrol. For pre-incubation experiments, cells were incubated with either incubation medium or 10 microM hydroxycinnamate or resveratrol for 24 hours followed by up to 24 hours PQ incubation. Cell viability and death were measured using the MTT and lactate dehydrogenase (LDH) assays, respectively. Furthermore, the antioxidant capacities of the hydroxycinnamates and resveratrol were investigated using the ABTS radical scavenging assay and their internalization into renal cells over 24 hours was measured using LC-MS. Results: Hydroxycinnamates and resveratrol demonstrated minimal toxicity up to 5mM after 24 hours. No significant protection was observed when NRK-52E cells were co-incubated with hydroxycinnamates or resveratrol and PQ together for 24 hours. In contrast, significant protection against PQ toxicity was demonstrated after 24 hour pre-incubation with a protective hierarchy of (1) ferulic acid,(2) meta-coumaric and (3) resveratrol after 24 hours when cell viability was measured (Table 1). A similar profile of protection was observed for cell death. Furthermore, these effects were paralleled by radical scavenging activity and internalization data.
Conclusions: This in vitro investigation demonstrates that pre-treatment of proximal tubular cells with hydroxycinnamates or resveratrol provides significant protection against nephrotoxic oxidant injury with the hydroxycinnamate ferulic acid providing the greatest protection.
Original languageEnglish
Publication statusPublished - 1 Jul 2018
EventThe 18th World Congress of Basic and Clinical Pharmacology - Kyoto International Conference Center, Kyoto, Japan
Duration: 1 Jul 20186 Jul 2018
Conference number: 18th


ConferenceThe 18th World Congress of Basic and Clinical Pharmacology
Abbreviated titleWCP2018


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