Control of the beat cycle of respiratory tract cilia by Ca2+and cAMP

Alison Lansley, Michael J. Sanderson, Ellen R. Dirksen

Research output: Contribution to journalArticle

Abstract

Beat frequency and the duration of the constituent recovery, effective, and rest phases of the beat cycle of respiratory tract cilia were measured photoelectronically before and after manipulation with ionomycin or isoproterenol. Both ionomycin, acting by increasing intracellular Ca2+, and isoproterenol, acting by elevating intracellular adenosine 3',5'-cyclic monophosphate (cAMP), increased beat frequency by reducing the duration of the three phases of the ciliary beat cycle in a similar manner. The addition of increasing concentrations of ATP to ciliated cells permeabilized by exposure to saponin caused a pattern of phase reduction indistinguishable from that observed in whole cells. The beat frequency of permeabilized cells was slower than that of whole cells and insensitive to changes in Ca2+ and cAMP. Ca2+ and cAMP may regulate ciliary beat frequency by acting at a common site within intact cells, possibly regulating the rate at which the axoneme can use ATP or the availability of ATP to the axoneme
Original languageEnglish
Pages (from-to)232-242
Number of pages11
JournalAJP - Lung Cellular and Molecular Physiology
Volume263
Issue number2
Publication statusPublished - 1 Aug 1992

Fingerprint

cilia
respiratory system
calcium
cells
duration
cyclic AMP
saponins

Cite this

Lansley, Alison ; Sanderson, Michael J. ; Dirksen, Ellen R. / Control of the beat cycle of respiratory tract cilia by Ca2+and cAMP. In: AJP - Lung Cellular and Molecular Physiology. 1992 ; Vol. 263, No. 2. pp. 232-242.
@article{a3d51ed929574d0686d145911a1ddf98,
title = "Control of the beat cycle of respiratory tract cilia by Ca2+and cAMP",
abstract = "Beat frequency and the duration of the constituent recovery, effective, and rest phases of the beat cycle of respiratory tract cilia were measured photoelectronically before and after manipulation with ionomycin or isoproterenol. Both ionomycin, acting by increasing intracellular Ca2+, and isoproterenol, acting by elevating intracellular adenosine 3',5'-cyclic monophosphate (cAMP), increased beat frequency by reducing the duration of the three phases of the ciliary beat cycle in a similar manner. The addition of increasing concentrations of ATP to ciliated cells permeabilized by exposure to saponin caused a pattern of phase reduction indistinguishable from that observed in whole cells. The beat frequency of permeabilized cells was slower than that of whole cells and insensitive to changes in Ca2+ and cAMP. Ca2+ and cAMP may regulate ciliary beat frequency by acting at a common site within intact cells, possibly regulating the rate at which the axoneme can use ATP or the availability of ATP to the axoneme",
author = "Alison Lansley and Sanderson, {Michael J.} and Dirksen, {Ellen R.}",
year = "1992",
month = "8",
day = "1",
language = "English",
volume = "263",
pages = "232--242",
journal = "AJP - Lung Cellular and Molecular Physiology",
issn = "1040-0605",
number = "2",

}

Control of the beat cycle of respiratory tract cilia by Ca2+and cAMP. / Lansley, Alison; Sanderson, Michael J.; Dirksen, Ellen R.

In: AJP - Lung Cellular and Molecular Physiology, Vol. 263, No. 2, 01.08.1992, p. 232-242.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Control of the beat cycle of respiratory tract cilia by Ca2+and cAMP

AU - Lansley, Alison

AU - Sanderson, Michael J.

AU - Dirksen, Ellen R.

PY - 1992/8/1

Y1 - 1992/8/1

N2 - Beat frequency and the duration of the constituent recovery, effective, and rest phases of the beat cycle of respiratory tract cilia were measured photoelectronically before and after manipulation with ionomycin or isoproterenol. Both ionomycin, acting by increasing intracellular Ca2+, and isoproterenol, acting by elevating intracellular adenosine 3',5'-cyclic monophosphate (cAMP), increased beat frequency by reducing the duration of the three phases of the ciliary beat cycle in a similar manner. The addition of increasing concentrations of ATP to ciliated cells permeabilized by exposure to saponin caused a pattern of phase reduction indistinguishable from that observed in whole cells. The beat frequency of permeabilized cells was slower than that of whole cells and insensitive to changes in Ca2+ and cAMP. Ca2+ and cAMP may regulate ciliary beat frequency by acting at a common site within intact cells, possibly regulating the rate at which the axoneme can use ATP or the availability of ATP to the axoneme

AB - Beat frequency and the duration of the constituent recovery, effective, and rest phases of the beat cycle of respiratory tract cilia were measured photoelectronically before and after manipulation with ionomycin or isoproterenol. Both ionomycin, acting by increasing intracellular Ca2+, and isoproterenol, acting by elevating intracellular adenosine 3',5'-cyclic monophosphate (cAMP), increased beat frequency by reducing the duration of the three phases of the ciliary beat cycle in a similar manner. The addition of increasing concentrations of ATP to ciliated cells permeabilized by exposure to saponin caused a pattern of phase reduction indistinguishable from that observed in whole cells. The beat frequency of permeabilized cells was slower than that of whole cells and insensitive to changes in Ca2+ and cAMP. Ca2+ and cAMP may regulate ciliary beat frequency by acting at a common site within intact cells, possibly regulating the rate at which the axoneme can use ATP or the availability of ATP to the axoneme

M3 - Article

VL - 263

SP - 232

EP - 242

JO - AJP - Lung Cellular and Molecular Physiology

JF - AJP - Lung Cellular and Molecular Physiology

SN - 1040-0605

IS - 2

ER -