Constructing tissue regeneration scaffolds using marine biomolecules

Matthew Illsley, Cressida Bowyer, Sylvia Colliec-Jouault, Peter Dubruel, Diana-Maria Dragusin, Bertrand Thollas, Iain Allan

Research output: Contribution to conferenceOtherResearch

Abstract

Severe burn injuries pose an immediate threat to life. The wound must be covered to prevent dehydration and infection of the affected area. Following these critical measures, implantation of dermal regeneration scaffolds following debridement of the wound site has become an accepted means of regenerating the lost dermal tissue at the site of insult. Current dermal tissue regeneration matrices are expensive and are generally derived from mammalian tissue. Method: We have been examining the use of fish gelatin and agarose as naturally gelling materials of marine origin as alternative ingredients in dermal tissue regeneration scaffolds. By formulating these materials into cryogels we have produced highly macroporous, morphologically uniform and elastic materials. Results: A range of gel formulations with varying degrees of cross-linking were examined for their rheological properties. The most favoured formulation was assessed for its ability to accommodate the attachment and proliferation of human dermal fibroblasts in cell culture over a defined time interval. Confocal laser scanning microscopy revealed that the cells readily infiltrated and proliferated within the scaffolds. Discussion: Cryogel formulations containing cross-linked fish gelatin and agarose show promise as dermal tissue replacement materials.
Original languageEnglish
Pages0-0
Number of pages1
Publication statusPublished - 25 Sep 2014
Event1st international conference on repair, regeneration and reconstruction, Royal Institution of Great Britain, London, September 2014 - Royal Institution of Great Britain
Duration: 25 Sep 2014 → …

Conference

Conference1st international conference on repair, regeneration and reconstruction, Royal Institution of Great Britain, London, September 2014
Period25/09/14 → …

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Tissue Scaffolds
Regeneration
Skin
Cryogels
Gelatin
Sepharose
Wounds and Injuries
Fishes
Debridement
Dehydration
Confocal Microscopy
Cell Culture Techniques
Fibroblasts
Gels
Infection

Cite this

Illsley, M., Bowyer, C., Colliec-Jouault, S., Dubruel, P., Dragusin, D-M., Thollas, B., & Allan, I. (2014). Constructing tissue regeneration scaffolds using marine biomolecules. 0-0. 1st international conference on repair, regeneration and reconstruction, Royal Institution of Great Britain, London, September 2014, .
Illsley, Matthew ; Bowyer, Cressida ; Colliec-Jouault, Sylvia ; Dubruel, Peter ; Dragusin, Diana-Maria ; Thollas, Bertrand ; Allan, Iain. / Constructing tissue regeneration scaffolds using marine biomolecules. 1st international conference on repair, regeneration and reconstruction, Royal Institution of Great Britain, London, September 2014, .1 p.
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Illsley, M, Bowyer, C, Colliec-Jouault, S, Dubruel, P, Dragusin, D-M, Thollas, B & Allan, I 2014, 'Constructing tissue regeneration scaffolds using marine biomolecules' 1st international conference on repair, regeneration and reconstruction, Royal Institution of Great Britain, London, September 2014, 25/09/14, pp. 0-0.

Constructing tissue regeneration scaffolds using marine biomolecules. / Illsley, Matthew; Bowyer, Cressida; Colliec-Jouault, Sylvia; Dubruel, Peter; Dragusin, Diana-Maria; Thollas, Bertrand; Allan, Iain.

2014. 0-0 1st international conference on repair, regeneration and reconstruction, Royal Institution of Great Britain, London, September 2014, .

Research output: Contribution to conferenceOtherResearch

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T1 - Constructing tissue regeneration scaffolds using marine biomolecules

AU - Illsley, Matthew

AU - Bowyer, Cressida

AU - Colliec-Jouault, Sylvia

AU - Dubruel, Peter

AU - Dragusin, Diana-Maria

AU - Thollas, Bertrand

AU - Allan, Iain

PY - 2014/9/25

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N2 - Severe burn injuries pose an immediate threat to life. The wound must be covered to prevent dehydration and infection of the affected area. Following these critical measures, implantation of dermal regeneration scaffolds following debridement of the wound site has become an accepted means of regenerating the lost dermal tissue at the site of insult. Current dermal tissue regeneration matrices are expensive and are generally derived from mammalian tissue. Method: We have been examining the use of fish gelatin and agarose as naturally gelling materials of marine origin as alternative ingredients in dermal tissue regeneration scaffolds. By formulating these materials into cryogels we have produced highly macroporous, morphologically uniform and elastic materials. Results: A range of gel formulations with varying degrees of cross-linking were examined for their rheological properties. The most favoured formulation was assessed for its ability to accommodate the attachment and proliferation of human dermal fibroblasts in cell culture over a defined time interval. Confocal laser scanning microscopy revealed that the cells readily infiltrated and proliferated within the scaffolds. Discussion: Cryogel formulations containing cross-linked fish gelatin and agarose show promise as dermal tissue replacement materials.

AB - Severe burn injuries pose an immediate threat to life. The wound must be covered to prevent dehydration and infection of the affected area. Following these critical measures, implantation of dermal regeneration scaffolds following debridement of the wound site has become an accepted means of regenerating the lost dermal tissue at the site of insult. Current dermal tissue regeneration matrices are expensive and are generally derived from mammalian tissue. Method: We have been examining the use of fish gelatin and agarose as naturally gelling materials of marine origin as alternative ingredients in dermal tissue regeneration scaffolds. By formulating these materials into cryogels we have produced highly macroporous, morphologically uniform and elastic materials. Results: A range of gel formulations with varying degrees of cross-linking were examined for their rheological properties. The most favoured formulation was assessed for its ability to accommodate the attachment and proliferation of human dermal fibroblasts in cell culture over a defined time interval. Confocal laser scanning microscopy revealed that the cells readily infiltrated and proliferated within the scaffolds. Discussion: Cryogel formulations containing cross-linked fish gelatin and agarose show promise as dermal tissue replacement materials.

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Illsley M, Bowyer C, Colliec-Jouault S, Dubruel P, Dragusin D-M, Thollas B et al. Constructing tissue regeneration scaffolds using marine biomolecules. 2014. 1st international conference on repair, regeneration and reconstruction, Royal Institution of Great Britain, London, September 2014, .