Conservation of cancer genes in the marine invertebrate Mytilus edulis

Corina Ciocan, Jeanette Rotchell

Research output: Contribution to journalArticle

Abstract

Mussels are susceptible to a wide range of environmental
toxicants, including carcinogens, and thus are often
employed as bioindicator species. To elucidate the molecular
aetiology of such neoplastic damage, we have cloned
Mytilus edulis homologues of the vertebrate ras protooncogene,
and p53 tumor suppressor gene. The M. edulis
ras cDNA encodes a predicted protein of 184 amino
acids. The DNA sequence analysis with vertebrate ras
sequences demonstrates that the M. edulis ras cDNA is
highly conserved in regions of functional importance, including
mutational hot spots. The partial p53 sequence also
demonstrates that M. edulis p53 is highly conserved in
two regions of functional importance and that these regions
also include four of the five mutational hot spots for this
gene. In contrast, the M. edulis p53 sequence shows little
similarity to the other published invertebrate p53-like
sequences. The cancer gene sequences characterized
herein will allow development of specific biomarkers of
genotoxic damage.
Original languageEnglish
Pages (from-to)3029-3033
Number of pages3033
JournalEnvironmental Science and Technology
Volume39
Issue number9
DOIs
Publication statusPublished - 11 Mar 2005

Fingerprint

Mytilus edulis
invertebrates
neoplasms
vertebrates
proto-oncogenes
nucleotide sequences
tumor suppressor genes
genes
carcinogens
indicator species
mussels
biomarkers
sequence analysis
proteins

Keywords

  • cancer genes
  • molecular biology
  • marine invertebrates

Cite this

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abstract = "Mussels are susceptible to a wide range of environmentaltoxicants, including carcinogens, and thus are oftenemployed as bioindicator species. To elucidate the molecularaetiology of such neoplastic damage, we have clonedMytilus edulis homologues of the vertebrate ras protooncogene,and p53 tumor suppressor gene. The M. edulisras cDNA encodes a predicted protein of 184 aminoacids. The DNA sequence analysis with vertebrate rassequences demonstrates that the M. edulis ras cDNA ishighly conserved in regions of functional importance, includingmutational hot spots. The partial p53 sequence alsodemonstrates that M. edulis p53 is highly conserved intwo regions of functional importance and that these regionsalso include four of the five mutational hot spots for thisgene. In contrast, the M. edulis p53 sequence shows littlesimilarity to the other published invertebrate p53-likesequences. The cancer gene sequences characterizedherein will allow development of specific biomarkers ofgenotoxic damage.",
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Conservation of cancer genes in the marine invertebrate Mytilus edulis. / Ciocan, Corina; Rotchell, Jeanette.

In: Environmental Science and Technology, Vol. 39, No. 9, 11.03.2005, p. 3029-3033.

Research output: Contribution to journalArticle

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AB - Mussels are susceptible to a wide range of environmentaltoxicants, including carcinogens, and thus are oftenemployed as bioindicator species. To elucidate the molecularaetiology of such neoplastic damage, we have clonedMytilus edulis homologues of the vertebrate ras protooncogene,and p53 tumor suppressor gene. The M. edulisras cDNA encodes a predicted protein of 184 aminoacids. The DNA sequence analysis with vertebrate rassequences demonstrates that the M. edulis ras cDNA ishighly conserved in regions of functional importance, includingmutational hot spots. The partial p53 sequence alsodemonstrates that M. edulis p53 is highly conserved intwo regions of functional importance and that these regionsalso include four of the five mutational hot spots for thisgene. In contrast, the M. edulis p53 sequence shows littlesimilarity to the other published invertebrate p53-likesequences. The cancer gene sequences characterizedherein will allow development of specific biomarkers ofgenotoxic damage.

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