Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

F.M. Plieva; Irina Savina; S. Deraz; J. Andersson; I.Y. Galaev; B. Mattiasson

doi:10.1016/j.jchromb.2004.01.050

Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

F.M. Plieva, Irina Savina, S. Deraz, J. Andersson, I.Y. Galaev, B. Mattiasson

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N′-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5–100 μm. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel.

Original language	English
Pages (from-to)	129-137
Number of pages	9
Journal	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume	807
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2004.01.050
Publication status	Published - 25 Jul 2004

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@article{6773400380174849b85bb50b25a40d9c,

title = "Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles",

abstract = "Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N′-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5–100 μm. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel.",

author = "F.M. Plieva and Irina Savina and S. Deraz and J. Andersson and I.Y. Galaev and B. Mattiasson",

year = "2004",

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doi = "10.1016/j.jchromb.2004.01.050",

language = "English",

volume = "807",

pages = "129--137",

journal = "Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

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Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles. / Plieva, F.M.; Savina, Irina; Deraz, S. et al.
In: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Vol. 807, No. 1, 25.07.2004, p. 129-137.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

AU - Plieva, F.M.

AU - Savina, Irina

AU - Deraz, S.

AU - Andersson, J.

AU - Galaev, I.Y.

AU - Mattiasson, B.

PY - 2004/7/25

Y1 - 2004/7/25

N2 - Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N′-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5–100 μm. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel.

AB - Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N′-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5–100 μm. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel.

U2 - 10.1016/j.jchromb.2004.01.050

DO - 10.1016/j.jchromb.2004.01.050

M3 - Article

SN - 1570-0232

VL - 807

SP - 129

EP - 137

JO - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

IS - 1

ER -

Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

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