Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles

F.M. Plieva, Irina Savina, S. Deraz, J. Andersson, I.Y. Galaev, B. Mattiasson

Research output: Contribution to journalArticle

Abstract

Supermacroporous monolithic acrylamide (AAm)-based cryogels were prepared by radical cryo-polymerizaton (polymerization in the moderately frozen system) of AAm with functional monomers and cross-linker N,N′-methylene-bis-acrylamide (MBAAm). Electron microscopy studies revealed supermacroporous structure of the developed cryogels with pore size of 5–100 μm. Cryogel porosity depended on cryo-polymerization conditions. More than 90% of the monolithic bed volume is the interconnected supermacropores filled with water and less than 10% of the monolithic volume is pore walls. The total protein binding capacity (lysozyme in the case of immobilized metal affinity chromatography (IMAC) column and bovine serum albumin (BSA) in the case of anion-exchange (AE) column) was independent of the flow rates till 600 cm/h. Chromatographic behavior of E. coli cells when a cell suspension was applied to ion-exchange cryogel columns depended on both the density of functional ligand and the porosity of the cryogel.
Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalJournal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Volume807
Issue number1
DOIs
Publication statusPublished - 25 Jul 2004

Fingerprint Dive into the research topics of 'Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles'. Together they form a unique fingerprint.

  • Cite this