Cell-derived extracellular vesicles can be used as a biomarker reservoir for glioblastoma tumor subtyping

Rosemary Lane, Thomas Simon, Marian Vintu, Benjamin Solkin, Barbara Koch, Nicolas Stewart, Graeme Benstead-Hume, Frances Pearl, Giles Critchley, Justin Stebbing, Georgios Giamas

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Glioblastoma (GBM) is one of the most aggressive solid tumors for which treatment options and biomarkers are limited. Small extracellular vesicles (sEVs) produced by both GBM and stromal cells are central in the inter-cellular communication that is taking place in the tumor bulk. As tumor sEVs are accessible in biofluids, recent reports have suggested that sEVs contain valuable biomarkers for GBM patient diagnosis and follow-up. The aim of the current study was to describe the protein content of sEVs produced by different GBM cell lines and patient-derived stem cells. Our results reveal that the content of the sEVs mirrors the phenotypic signature of the respective GBM cells, leading to the description of potential informative sEV-associated biomarkers for GBM subtyping, such as CD44. Overall, these data could assist future GBM in vitro studies and provide insights for the development of new diagnostic and therapeutic methods as well as personalized treatment strategies.
Original languageEnglish
Article number315
JournalCommunications Biology
Volume2
Issue number1
DOIs
Publication statusPublished - 19 Aug 2019

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Glioblastoma
Biomarkers
Neoplasms
Stromal Cells
Extracellular Vesicles
Stem Cells
Therapeutics
Communication
Cell Line
Proteins

Bibliographical note

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Cite this

Lane, Rosemary ; Simon, Thomas ; Vintu, Marian ; Solkin, Benjamin ; Koch, Barbara ; Stewart, Nicolas ; Benstead-Hume, Graeme ; Pearl, Frances ; Critchley, Giles ; Stebbing, Justin ; Giamas, Georgios. / Cell-derived extracellular vesicles can be used as a biomarker reservoir for glioblastoma tumor subtyping. In: Communications Biology. 2019 ; Vol. 2, No. 1.
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Lane, R, Simon, T, Vintu, M, Solkin, B, Koch, B, Stewart, N, Benstead-Hume, G, Pearl, F, Critchley, G, Stebbing, J & Giamas, G 2019, 'Cell-derived extracellular vesicles can be used as a biomarker reservoir for glioblastoma tumor subtyping', Communications Biology, vol. 2, no. 1, 315. https://doi.org/10.1038/s42003-019-0560-x

Cell-derived extracellular vesicles can be used as a biomarker reservoir for glioblastoma tumor subtyping. / Lane, Rosemary; Simon, Thomas; Vintu, Marian; Solkin, Benjamin; Koch, Barbara; Stewart, Nicolas; Benstead-Hume, Graeme; Pearl, Frances; Critchley, Giles; Stebbing, Justin; Giamas, Georgios.

In: Communications Biology, Vol. 2, No. 1, 315, 19.08.2019.

Research output: Contribution to journalArticleResearchpeer-review

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