Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers

Inga Zeisset; T.J.C. Beebee

doi:10.1007/s12686-012-9770-y

Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers

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Abstract

We characterized the complete second exonwith adjacent introns of MHC class II beta genes in theanurans Bufo calamita, B. bufo and B. viridis. Introns wereremarkably conserved among species. There were at leasttwo loci (A and B), which we amplified with differentforward primers but identical reverse primers. Locus A hadlow diversity whereas locus B exhibited 42 alleles inB. calamita and 14 in B. bufo. We detected evidence ofpositive selection at antigen binding sites in both thesespecies and demonstrated expression of locus B in B.calamita.

Original language	English
Journal	Conservation Genetics Resources
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/s12686-012-9770-y
Publication status	Published - 1 Mar 2013

Bibliographical note

Keywords

MHC
Beta chain
Bufo calamita
Bufo bufo
Bufo viridis

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title = "Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers",

abstract = "We characterized the complete second exonwith adjacent introns of MHC class II beta genes in theanurans Bufo calamita, B. bufo and B. viridis. Introns wereremarkably conserved among species. There were at leasttwo loci (A and B), which we amplified with differentforward primers but identical reverse primers. Locus A hadlow diversity whereas locus B exhibited 42 alleles inB. calamita and 14 in B. bufo. We detected evidence ofpositive selection at antigen binding sites in both thesespecies and demonstrated expression of locus B in B.calamita.",

keywords = "MHC, Beta chain, Bufo calamita, Bufo bufo, Bufo viridis",

author = "Inga Zeisset and T.J.C. Beebee",

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T1 - Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers

AU - Zeisset, Inga

AU - Beebee, T.J.C.

PY - 2013/3/1

Y1 - 2013/3/1

N2 - We characterized the complete second exonwith adjacent introns of MHC class II beta genes in theanurans Bufo calamita, B. bufo and B. viridis. Introns wereremarkably conserved among species. There were at leasttwo loci (A and B), which we amplified with differentforward primers but identical reverse primers. Locus A hadlow diversity whereas locus B exhibited 42 alleles inB. calamita and 14 in B. bufo. We detected evidence ofpositive selection at antigen binding sites in both thesespecies and demonstrated expression of locus B in B.calamita.

AB - We characterized the complete second exonwith adjacent introns of MHC class II beta genes in theanurans Bufo calamita, B. bufo and B. viridis. Introns wereremarkably conserved among species. There were at leasttwo loci (A and B), which we amplified with differentforward primers but identical reverse primers. Locus A hadlow diversity whereas locus B exhibited 42 alleles inB. calamita and 14 in B. bufo. We detected evidence ofpositive selection at antigen binding sites in both thesespecies and demonstrated expression of locus B in B.calamita.

KW - MHC

KW - Beta chain

KW - Bufo calamita

KW - Bufo bufo

KW - Bufo viridis

U2 - 10.1007/s12686-012-9770-y

DO - 10.1007/s12686-012-9770-y

M3 - Article

SN - 1877-7252

VL - 5

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

IS - 1

ER -

Bufo MHC class II loci with conserved introns flanking exon 2: cross-species amplification with common primers

Abstract

Bibliographical note

Keywords

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