Assessing the in vitro biocompatibility of a novel carbon device for the treatment of sepsis

Susan Sandeman; Carol Howell; Gary Phillips; Andrew Lloyd; J.G. Davies; Sergey Mikhalovsky; S.R. Tennison; A.P. Rawlinson; O.P. Kozynchenko; H.L.H. Owen; J.D.S. Gaylor; J.J. Rouse; J.M. Courtney

doi:10.1016/j.biomaterials.2005.05.019

Assessing the in vitro biocompatibility of a novel carbon device for the treatment of sepsis

Susan Sandeman, Carol Howell, Gary Phillips, Andrew Lloyd, J.G. Davies, Sergey Mikhalovsky, S.R. Tennison, A.P. Rawlinson, O.P. Kozynchenko, H.L.H. Owen, J.D.S. Gaylor, J.J. Rouse, J.M. Courtney

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of the present study was to conduct a preliminary investigation into the blood biocompatibility of a novel, uncoated carbon for use in a filtration/adsorption device for the treatment of sepsis. Carbon well prototypes were manufactured from phenol–formaldehyde–aniline-based pyrolysed carbons using monolithic polymer technology. Inflammatory blood cell and plasma protein mediation of the inflammatory response were evaluated using the novel carbon prototypes and compared with dialyser membrane and tissue culture plate controls. Assays determining monocyte and granulocyte adhesion, platelet adhesion and activation, granulocyte activation and complement activation were performed. Preliminary findings suggest an adsorptive but passivating carbon surface. Moderate levels of monocyte and granulocytes adhesion were seen in conjunction with adsorption of plasma proteins to the carbon surface. Activation of granulocyte and adherent platelets was not detected and the complement cascade was not activated by the carbons, indicating a surface compatible with blood contact. The results support the further development of the proposed carbon-based device for the treatment of sepsis.

Original language	English
Pages (from-to)	7124-7131
Number of pages	8
Journal	Biomaterials
Volume	26
Issue number	34
DOIs	https://doi.org/10.1016/j.biomaterials.2005.05.019
Publication status	Published - 1 Dec 2005

Keywords

Blood compatibility
Carbon
Cell adhesion/activation
Complement
Sepsis

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Sandeman, S., Howell, C., Phillips, G., Lloyd, A., Davies, J. G., Mikhalovsky, S., Tennison, S. R., Rawlinson, A. P., Kozynchenko, O. P., Owen, H. L. H., Gaylor, J. D. S., Rouse, J. J., & Courtney, J. M. (2005). Assessing the in vitro biocompatibility of a novel carbon device for the treatment of sepsis. Biomaterials, 26(34), 7124-7131. https://doi.org/10.1016/j.biomaterials.2005.05.019

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abstract = "The aim of the present study was to conduct a preliminary investigation into the blood biocompatibility of a novel, uncoated carbon for use in a filtration/adsorption device for the treatment of sepsis. Carbon well prototypes were manufactured from phenol–formaldehyde–aniline-based pyrolysed carbons using monolithic polymer technology. Inflammatory blood cell and plasma protein mediation of the inflammatory response were evaluated using the novel carbon prototypes and compared with dialyser membrane and tissue culture plate controls. Assays determining monocyte and granulocyte adhesion, platelet adhesion and activation, granulocyte activation and complement activation were performed. Preliminary findings suggest an adsorptive but passivating carbon surface. Moderate levels of monocyte and granulocytes adhesion were seen in conjunction with adsorption of plasma proteins to the carbon surface. Activation of granulocyte and adherent platelets was not detected and the complement cascade was not activated by the carbons, indicating a surface compatible with blood contact. The results support the further development of the proposed carbon-based device for the treatment of sepsis.",

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AU - Lloyd, Andrew

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AU - Mikhalovsky, Sergey

AU - Tennison, S.R.

AU - Rawlinson, A.P.

AU - Kozynchenko, O.P.

AU - Owen, H.L.H.

AU - Gaylor, J.D.S.

AU - Rouse, J.J.

AU - Courtney, J.M.

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N2 - The aim of the present study was to conduct a preliminary investigation into the blood biocompatibility of a novel, uncoated carbon for use in a filtration/adsorption device for the treatment of sepsis. Carbon well prototypes were manufactured from phenol–formaldehyde–aniline-based pyrolysed carbons using monolithic polymer technology. Inflammatory blood cell and plasma protein mediation of the inflammatory response were evaluated using the novel carbon prototypes and compared with dialyser membrane and tissue culture plate controls. Assays determining monocyte and granulocyte adhesion, platelet adhesion and activation, granulocyte activation and complement activation were performed. Preliminary findings suggest an adsorptive but passivating carbon surface. Moderate levels of monocyte and granulocytes adhesion were seen in conjunction with adsorption of plasma proteins to the carbon surface. Activation of granulocyte and adherent platelets was not detected and the complement cascade was not activated by the carbons, indicating a surface compatible with blood contact. The results support the further development of the proposed carbon-based device for the treatment of sepsis.

AB - The aim of the present study was to conduct a preliminary investigation into the blood biocompatibility of a novel, uncoated carbon for use in a filtration/adsorption device for the treatment of sepsis. Carbon well prototypes were manufactured from phenol–formaldehyde–aniline-based pyrolysed carbons using monolithic polymer technology. Inflammatory blood cell and plasma protein mediation of the inflammatory response were evaluated using the novel carbon prototypes and compared with dialyser membrane and tissue culture plate controls. Assays determining monocyte and granulocyte adhesion, platelet adhesion and activation, granulocyte activation and complement activation were performed. Preliminary findings suggest an adsorptive but passivating carbon surface. Moderate levels of monocyte and granulocytes adhesion were seen in conjunction with adsorption of plasma proteins to the carbon surface. Activation of granulocyte and adherent platelets was not detected and the complement cascade was not activated by the carbons, indicating a surface compatible with blood contact. The results support the further development of the proposed carbon-based device for the treatment of sepsis.

KW - Blood compatibility

KW - Carbon

KW - Cell adhesion/activation

KW - Complement

KW - Sepsis

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DO - 10.1016/j.biomaterials.2005.05.019

M3 - Article

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JO - Biomaterials

JF - Biomaterials

IS - 34

ER -

Assessing the in vitro biocompatibility of a novel carbon device for the treatment of sepsis

Abstract

Keywords

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