Argatroban immobilization on Cu-modified PVC and PU

Liana Azizova, Lyuba Mikhalovska, Volodymyr Chernyshenko

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNConference contribution with ISSN or ISBN

Abstract

Thrombosis induced by biomaterials after their contact with blood is a main reason of medical device failure. To make material surface more thromboresistant different approaches have been undertaken. NO generating biomaterial has proven to play a crucial role in the prevention of thrombosis by inhibiting the platelets activation/adhesion. However, immobilization of the direct thrombin inhibitors onto material surface makes material more thromboresistant by preventing thrombin-mediated blood clotting. The aim of this research was to immobilize argatroban a direct thrombin inhibitor with reliable and predictable anticoagulant effect onto PVC and PU polymers. Both polymers were first imprinted with Cu ions for the catalytic generation of NO (this research was reported earlier). Argatroban was immobilized on the Cu-modified PVC and PU using the polydopamine ad-layer via the Michael addition/Schiff base reaction. The amount of argatroban bound to the polymer surface was measured (spectrophotometric determination at 334 nm) as 11.92 nmol/cm2 on PVC and 13.10 nmol/ cm2 on PU surface. Assay using thrombin-specific chromogenic substrate was performed to evaluate the thrombin inhibition capacity of argatroban-modified polymers. It was found that both Argatroban-modified polymers inhibit thrombin activity in PBS. In order to confirm the NO generation catalyzed by Cu/Arg-modified PVC and PU samples after incubation with 100 ?M GSNO/GSH in the PBS during 1h was examined using ArrowSTRAIGHT??? nitric oxide measurement system (Lazar Research Laboratories, Los Angeles, CA, USA). The Cu/Arg-modified PVC and PU generate NO with the rate 1.27-1.66??1010 mole/cm2???min which is within the physiological level. From the data obtained it???s possible to conclude, that immobilization of Argatroban to the Cu-modified polymers showed combine abilities: i) generate NO caused by Cu ions and ii) have capacity to inhibit thrombin formed in the blood via surface immobilized argatroban.
Original languageEnglish
Title of host publicationProceedings of 4th Annual Conference and Expo On Biomaterials 2019
Place of PublicationLondon
Pages33-34
Volume9
DOIs
Publication statusAccepted/In press - 31 Dec 2019
Event4th Annual Conference and Expo on Biomaterials - London, United Kingdom
Duration: 25 Feb 201926 Feb 2019
https://biomaterials.insightconferences.com/

Publication series

NameJournal of Biotechnology & Biomaterials
ISSN (Print)2155-952X

Conference

Conference4th Annual Conference and Expo on Biomaterials
CountryUnited Kingdom
CityLondon
Period25/02/1926/02/19
Internet address

Fingerprint

Polyvinyl Chloride
Polymers
Thrombin
Blood
Antithrombins
Biocompatible Materials
Ions
Chromogenic Compounds
Schiff Bases
Research laboratories
Platelets
argatroban
Anticoagulants
Assays
Nitric Oxide
Adhesion
Chemical activation

Cite this

Azizova, L., Mikhalovska, L., & Chernyshenko, V. (Accepted/In press). Argatroban immobilization on Cu-modified PVC and PU. In Proceedings of 4th Annual Conference and Expo On Biomaterials 2019 (Vol. 9, pp. 33-34). (Journal of Biotechnology & Biomaterials). London. https://doi.org/10.4172/2155-952X-C1-112
Azizova, Liana ; Mikhalovska, Lyuba ; Chernyshenko, Volodymyr. / Argatroban immobilization on Cu-modified PVC and PU. Proceedings of 4th Annual Conference and Expo On Biomaterials 2019. Vol. 9 London, 2019. pp. 33-34 (Journal of Biotechnology & Biomaterials).
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Azizova, L, Mikhalovska, L & Chernyshenko, V 2019, Argatroban immobilization on Cu-modified PVC and PU. in Proceedings of 4th Annual Conference and Expo On Biomaterials 2019. vol. 9, Journal of Biotechnology & Biomaterials, London, pp. 33-34, 4th Annual Conference and Expo on Biomaterials, London, United Kingdom, 25/02/19. https://doi.org/10.4172/2155-952X-C1-112

Argatroban immobilization on Cu-modified PVC and PU. / Azizova, Liana; Mikhalovska, Lyuba; Chernyshenko, Volodymyr.

Proceedings of 4th Annual Conference and Expo On Biomaterials 2019. Vol. 9 London, 2019. p. 33-34 (Journal of Biotechnology & Biomaterials).

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNConference contribution with ISSN or ISBN

TY - GEN

T1 - Argatroban immobilization on Cu-modified PVC and PU

AU - Azizova, Liana

AU - Mikhalovska, Lyuba

AU - Chernyshenko, Volodymyr

PY - 2019/12/31

Y1 - 2019/12/31

N2 - Thrombosis induced by biomaterials after their contact with blood is a main reason of medical device failure. To make material surface more thromboresistant different approaches have been undertaken. NO generating biomaterial has proven to play a crucial role in the prevention of thrombosis by inhibiting the platelets activation/adhesion. However, immobilization of the direct thrombin inhibitors onto material surface makes material more thromboresistant by preventing thrombin-mediated blood clotting. The aim of this research was to immobilize argatroban a direct thrombin inhibitor with reliable and predictable anticoagulant effect onto PVC and PU polymers. Both polymers were first imprinted with Cu ions for the catalytic generation of NO (this research was reported earlier). Argatroban was immobilized on the Cu-modified PVC and PU using the polydopamine ad-layer via the Michael addition/Schiff base reaction. The amount of argatroban bound to the polymer surface was measured (spectrophotometric determination at 334 nm) as 11.92 nmol/cm2 on PVC and 13.10 nmol/ cm2 on PU surface. Assay using thrombin-specific chromogenic substrate was performed to evaluate the thrombin inhibition capacity of argatroban-modified polymers. It was found that both Argatroban-modified polymers inhibit thrombin activity in PBS. In order to confirm the NO generation catalyzed by Cu/Arg-modified PVC and PU samples after incubation with 100 ?M GSNO/GSH in the PBS during 1h was examined using ArrowSTRAIGHT??? nitric oxide measurement system (Lazar Research Laboratories, Los Angeles, CA, USA). The Cu/Arg-modified PVC and PU generate NO with the rate 1.27-1.66??1010 mole/cm2???min which is within the physiological level. From the data obtained it???s possible to conclude, that immobilization of Argatroban to the Cu-modified polymers showed combine abilities: i) generate NO caused by Cu ions and ii) have capacity to inhibit thrombin formed in the blood via surface immobilized argatroban.

AB - Thrombosis induced by biomaterials after their contact with blood is a main reason of medical device failure. To make material surface more thromboresistant different approaches have been undertaken. NO generating biomaterial has proven to play a crucial role in the prevention of thrombosis by inhibiting the platelets activation/adhesion. However, immobilization of the direct thrombin inhibitors onto material surface makes material more thromboresistant by preventing thrombin-mediated blood clotting. The aim of this research was to immobilize argatroban a direct thrombin inhibitor with reliable and predictable anticoagulant effect onto PVC and PU polymers. Both polymers were first imprinted with Cu ions for the catalytic generation of NO (this research was reported earlier). Argatroban was immobilized on the Cu-modified PVC and PU using the polydopamine ad-layer via the Michael addition/Schiff base reaction. The amount of argatroban bound to the polymer surface was measured (spectrophotometric determination at 334 nm) as 11.92 nmol/cm2 on PVC and 13.10 nmol/ cm2 on PU surface. Assay using thrombin-specific chromogenic substrate was performed to evaluate the thrombin inhibition capacity of argatroban-modified polymers. It was found that both Argatroban-modified polymers inhibit thrombin activity in PBS. In order to confirm the NO generation catalyzed by Cu/Arg-modified PVC and PU samples after incubation with 100 ?M GSNO/GSH in the PBS during 1h was examined using ArrowSTRAIGHT??? nitric oxide measurement system (Lazar Research Laboratories, Los Angeles, CA, USA). The Cu/Arg-modified PVC and PU generate NO with the rate 1.27-1.66??1010 mole/cm2???min which is within the physiological level. From the data obtained it???s possible to conclude, that immobilization of Argatroban to the Cu-modified polymers showed combine abilities: i) generate NO caused by Cu ions and ii) have capacity to inhibit thrombin formed in the blood via surface immobilized argatroban.

U2 - 10.4172/2155-952X-C1-112

DO - 10.4172/2155-952X-C1-112

M3 - Conference contribution with ISSN or ISBN

VL - 9

T3 - Journal of Biotechnology & Biomaterials

SP - 33

EP - 34

BT - Proceedings of 4th Annual Conference and Expo On Biomaterials 2019

CY - London

ER -

Azizova L, Mikhalovska L, Chernyshenko V. Argatroban immobilization on Cu-modified PVC and PU. In Proceedings of 4th Annual Conference and Expo On Biomaterials 2019. Vol. 9. London. 2019. p. 33-34. (Journal of Biotechnology & Biomaterials). https://doi.org/10.4172/2155-952X-C1-112