Antioxidant properties and global metabolite screening ofthe probiotic yeast Saccharomyces cerevisiae var. boulardii

Suprama Datta, David Timson, Uday S. Annapure

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastro-intestinal disorders. In this paper, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of probiotic species, responses to different stress conditions and antioxidant capacity. A global metabolite profile was also developed in order to identify which therapeutically important secondary metabolites are produced. RESULTS: S. cerevisiae var. boulardii showed no significant difference in growth patterns but greater stress tolerance when compared with S. cerevisiae. It also demonstrated a 6-10-fold greater antioxidant potential (judged by the DPPH assay), with 70-fold higher total phenolic content and 20-fold higher total flavonoid content in the extracellular fraction. These features were clearly differentiated by principal component analysis and further elucidated by metabolite profiling. The extracellular fraction of the S. cerevisiae var. boulardii cultures was found to be rich in polyphenolic metabolites viz. vanillic acid, cinnamic acid, phenyl ethyl alcohol (rose oil), erythromycin, amphetamine and vitamin B6 which results in the antioxidant capacity of this strain. CONCLUSION: This study presents a new perspective of differentiating the two genetically related strains of yeast, Saccharomyces cerevisiae and Saccharomyces cerevisiae var. boulardii by assessing their metabolome fingerprints. In addition to the correlation of the phenotypic properties with the secretory metabolites of these two yeasts, the work also emphasizes the potential to exploit S. cerevisiae var. boulardii in the industrial production of these metabolites.
Original languageEnglish
Pages (from-to)3039-3049
Number of pages11
JournalJournal of the Science of Food and Agriculture
Volume97
Issue number9
DOIs
Publication statusPublished - 21 Nov 2016

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probiotics
Saccharomyces cerevisiae
metabolites
yeasts
screening
antioxidants
amphetamine
metabolome
vanillic acid
cinnamic acid
erythromycin
phenotypic correlation
pyridoxine
digestive system diseases
stress tolerance
secondary metabolites
stress response
polyphenols
principal component analysis
Rosa

Bibliographical note

This is the peer reviewed version of the following article: Datta, S., Timson, D. J. and Annapure, U. S. (2016), Antioxidant properties and global metabolite screening of the probiotic yeast Saccharomyces cerevisiae var. boulardii. J. Sci. Food Agric, which has been published in final form at doi:10.1002/jsfa.8147. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving

Keywords

  • Saccharomyces cerevisiae var. boulardii NCYC 3264, probiotic
  • Saccharomyces cerevisiae BY4742
  • stress-tolerance
  • antioxidant capacity
  • global metabolite profiling.

Cite this

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title = "Antioxidant properties and global metabolite screening ofthe probiotic yeast Saccharomyces cerevisiae var. boulardii",
abstract = "BACKGROUND: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastro-intestinal disorders. In this paper, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of probiotic species, responses to different stress conditions and antioxidant capacity. A global metabolite profile was also developed in order to identify which therapeutically important secondary metabolites are produced. RESULTS: S. cerevisiae var. boulardii showed no significant difference in growth patterns but greater stress tolerance when compared with S. cerevisiae. It also demonstrated a 6-10-fold greater antioxidant potential (judged by the DPPH assay), with 70-fold higher total phenolic content and 20-fold higher total flavonoid content in the extracellular fraction. These features were clearly differentiated by principal component analysis and further elucidated by metabolite profiling. The extracellular fraction of the S. cerevisiae var. boulardii cultures was found to be rich in polyphenolic metabolites viz. vanillic acid, cinnamic acid, phenyl ethyl alcohol (rose oil), erythromycin, amphetamine and vitamin B6 which results in the antioxidant capacity of this strain. CONCLUSION: This study presents a new perspective of differentiating the two genetically related strains of yeast, Saccharomyces cerevisiae and Saccharomyces cerevisiae var. boulardii by assessing their metabolome fingerprints. In addition to the correlation of the phenotypic properties with the secretory metabolites of these two yeasts, the work also emphasizes the potential to exploit S. cerevisiae var. boulardii in the industrial production of these metabolites.",
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Antioxidant properties and global metabolite screening ofthe probiotic yeast Saccharomyces cerevisiae var. boulardii. / Datta, Suprama; Timson, David; Annapure, Uday S.

In: Journal of the Science of Food and Agriculture, Vol. 97, No. 9, 21.11.2016, p. 3039-3049.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant properties and global metabolite screening ofthe probiotic yeast Saccharomyces cerevisiae var. boulardii

AU - Datta, Suprama

AU - Timson, David

AU - Annapure, Uday S.

N1 - This is the peer reviewed version of the following article: Datta, S., Timson, D. J. and Annapure, U. S. (2016), Antioxidant properties and global metabolite screening of the probiotic yeast Saccharomyces cerevisiae var. boulardii. J. Sci. Food Agric, which has been published in final form at doi:10.1002/jsfa.8147. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving

PY - 2016/11/21

Y1 - 2016/11/21

N2 - BACKGROUND: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastro-intestinal disorders. In this paper, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of probiotic species, responses to different stress conditions and antioxidant capacity. A global metabolite profile was also developed in order to identify which therapeutically important secondary metabolites are produced. RESULTS: S. cerevisiae var. boulardii showed no significant difference in growth patterns but greater stress tolerance when compared with S. cerevisiae. It also demonstrated a 6-10-fold greater antioxidant potential (judged by the DPPH assay), with 70-fold higher total phenolic content and 20-fold higher total flavonoid content in the extracellular fraction. These features were clearly differentiated by principal component analysis and further elucidated by metabolite profiling. The extracellular fraction of the S. cerevisiae var. boulardii cultures was found to be rich in polyphenolic metabolites viz. vanillic acid, cinnamic acid, phenyl ethyl alcohol (rose oil), erythromycin, amphetamine and vitamin B6 which results in the antioxidant capacity of this strain. CONCLUSION: This study presents a new perspective of differentiating the two genetically related strains of yeast, Saccharomyces cerevisiae and Saccharomyces cerevisiae var. boulardii by assessing their metabolome fingerprints. In addition to the correlation of the phenotypic properties with the secretory metabolites of these two yeasts, the work also emphasizes the potential to exploit S. cerevisiae var. boulardii in the industrial production of these metabolites.

AB - BACKGROUND: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastro-intestinal disorders. In this paper, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of probiotic species, responses to different stress conditions and antioxidant capacity. A global metabolite profile was also developed in order to identify which therapeutically important secondary metabolites are produced. RESULTS: S. cerevisiae var. boulardii showed no significant difference in growth patterns but greater stress tolerance when compared with S. cerevisiae. It also demonstrated a 6-10-fold greater antioxidant potential (judged by the DPPH assay), with 70-fold higher total phenolic content and 20-fold higher total flavonoid content in the extracellular fraction. These features were clearly differentiated by principal component analysis and further elucidated by metabolite profiling. The extracellular fraction of the S. cerevisiae var. boulardii cultures was found to be rich in polyphenolic metabolites viz. vanillic acid, cinnamic acid, phenyl ethyl alcohol (rose oil), erythromycin, amphetamine and vitamin B6 which results in the antioxidant capacity of this strain. CONCLUSION: This study presents a new perspective of differentiating the two genetically related strains of yeast, Saccharomyces cerevisiae and Saccharomyces cerevisiae var. boulardii by assessing their metabolome fingerprints. In addition to the correlation of the phenotypic properties with the secretory metabolites of these two yeasts, the work also emphasizes the potential to exploit S. cerevisiae var. boulardii in the industrial production of these metabolites.

KW - Saccharomyces cerevisiae var. boulardii NCYC 3264, probiotic

KW - Saccharomyces cerevisiae BY4742

KW - stress-tolerance

KW - antioxidant capacity

KW - global metabolite profiling.

U2 - 10.1002/jsfa.8147

DO - 10.1002/jsfa.8147

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SN - 0022-5142

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