Alcohol ethoxylates mediate their bacteriostatic effect by altering the cell membrane of Escherichia coli NCTC 8196

S.L. Moore; Stephen Denyer; Geoff Hanlon; C.J. Olliff; Alison Lansley; K. Rabone; M. Jones

doi:10.1016/j.ijantimicag.2006.08.023

Alcohol ethoxylates mediate their bacteriostatic effect by altering the cell membrane of Escherichia coli NCTC 8196

S.L. Moore, Stephen Denyer, Geoff Hanlon, C.J. Olliff, Alison Lansley, K. Rabone, M. Jones

Research output: Contribution to journal › Article › peer-review

Abstract

The minimum inhibitory concentration (MIC) of a homologous series of alcohol ethoxylates with the same head group size (E6) but differing in the number of carbon atoms in their ‘tail group’ from 10 to 16 was determined for Staphylococcus aureus NCTC 4163 and Escherichia coli NCTC 8196 using a turbidimetric assay. All the surfactants tested demonstrated bacteriostatic activity against both organisms. A tetrazolium assay showed that C14E6 and C16E6 had little effect on the membrane-bound dehydrogenase enzyme activity of E. coli NCTC 8196 compared with C10E6 and C12E6. C10E6 caused leakage both of K+ and nucleotides in a concentration-dependent manner above its MIC of 0.2 mM. C12E6 caused some leakage at concentrations below its MIC (0.12 mM).

Original language	English
Pages (from-to)	503-513
Number of pages	11
Journal	International Journal of Antimicrobial Agents
Volume	28
Issue number	6
DOIs	https://doi.org/10.1016/j.ijantimicag.2006.08.023
Publication status	Published - Dec 2006

Keywords

Alcohol ethoxylate
Non-ionic surfactant
Membrane-bound dehydrogenases
Bacterial membrane
Permeability
Escherichia coli
Antimicrobial

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http://dx.doi.org/10.1016/j.ijantimicag.2006.08.023Licence: Unspecified

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title = "Alcohol ethoxylates mediate their bacteriostatic effect by altering the cell membrane of Escherichia coli NCTC 8196",

abstract = "The minimum inhibitory concentration (MIC) of a homologous series of alcohol ethoxylates with the same head group size (E6) but differing in the number of carbon atoms in their {\textquoteleft}tail group{\textquoteright} from 10 to 16 was determined for Staphylococcus aureus NCTC 4163 and Escherichia coli NCTC 8196 using a turbidimetric assay. All the surfactants tested demonstrated bacteriostatic activity against both organisms. A tetrazolium assay showed that C14E6 and C16E6 had little effect on the membrane-bound dehydrogenase enzyme activity of E. coli NCTC 8196 compared with C10E6 and C12E6. C10E6 caused leakage both of K+ and nucleotides in a concentration-dependent manner above its MIC of 0.2 mM. C12E6 caused some leakage at concentrations below its MIC (0.12 mM).",

keywords = "Alcohol ethoxylate, Non-ionic surfactant, Membrane-bound dehydrogenases, Bacterial membrane, Permeability, Escherichia coli, Antimicrobial",

author = "S.L. Moore and Stephen Denyer and Geoff Hanlon and C.J. Olliff and Alison Lansley and K. Rabone and M. Jones",

year = "2006",

month = dec,

doi = "10.1016/j.ijantimicag.2006.08.023",

language = "English",

volume = "28",

pages = "503--513",

journal = "International Journal of Antimicrobial Agents",

issn = "0924-8579",

number = "6",

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TY - JOUR

T1 - Alcohol ethoxylates mediate their bacteriostatic effect by altering the cell membrane of Escherichia coli NCTC 8196

AU - Moore, S.L.

AU - Denyer, Stephen

AU - Hanlon, Geoff

AU - Olliff, C.J.

AU - Lansley, Alison

AU - Rabone, K.

AU - Jones, M.

PY - 2006/12

Y1 - 2006/12

N2 - The minimum inhibitory concentration (MIC) of a homologous series of alcohol ethoxylates with the same head group size (E6) but differing in the number of carbon atoms in their ‘tail group’ from 10 to 16 was determined for Staphylococcus aureus NCTC 4163 and Escherichia coli NCTC 8196 using a turbidimetric assay. All the surfactants tested demonstrated bacteriostatic activity against both organisms. A tetrazolium assay showed that C14E6 and C16E6 had little effect on the membrane-bound dehydrogenase enzyme activity of E. coli NCTC 8196 compared with C10E6 and C12E6. C10E6 caused leakage both of K+ and nucleotides in a concentration-dependent manner above its MIC of 0.2 mM. C12E6 caused some leakage at concentrations below its MIC (0.12 mM).

AB - The minimum inhibitory concentration (MIC) of a homologous series of alcohol ethoxylates with the same head group size (E6) but differing in the number of carbon atoms in their ‘tail group’ from 10 to 16 was determined for Staphylococcus aureus NCTC 4163 and Escherichia coli NCTC 8196 using a turbidimetric assay. All the surfactants tested demonstrated bacteriostatic activity against both organisms. A tetrazolium assay showed that C14E6 and C16E6 had little effect on the membrane-bound dehydrogenase enzyme activity of E. coli NCTC 8196 compared with C10E6 and C12E6. C10E6 caused leakage both of K+ and nucleotides in a concentration-dependent manner above its MIC of 0.2 mM. C12E6 caused some leakage at concentrations below its MIC (0.12 mM).

KW - Alcohol ethoxylate

KW - Non-ionic surfactant

KW - Membrane-bound dehydrogenases

KW - Bacterial membrane

KW - Permeability

KW - Escherichia coli

KW - Antimicrobial

U2 - 10.1016/j.ijantimicag.2006.08.023

DO - 10.1016/j.ijantimicag.2006.08.023

M3 - Article

SN - 0924-8579

VL - 28

SP - 503

EP - 513

JO - International Journal of Antimicrobial Agents

JF - International Journal of Antimicrobial Agents

IS - 6

ER -

Alcohol ethoxylates mediate their bacteriostatic effect by altering the cell membrane of Escherichia coli NCTC 8196

Abstract

Keywords

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