A switch in the mode of the sodium/calcium exchanger underlies an age-related increase in the slow afterhyperpolarization

Research output: Contribution to journalArticleResearchpeer-review

Abstract

During aging, the Ca2+-sensitive slow afterhyperpolarization (sAHP) of hippocampal neurons is known to increase in duration. This change has also been observed in the serotonergic cerebral giant cells (CGCs) of the pond snail Lymnaea stagnalis, but has yet to be characterized. In this article, we confirm that there is a reduction in firing rate, an increase in the duration of the sAHP, and an alteration in the strength and speed of spike frequency adaptation in the CGCs during aging, a finding that is compatible with an increase in the sAHP current. We go on to show that age-related changes in the kinetics of spike frequency adaptation are consistent with a reduction in Ca2+ clearance from the cell, which we confirm with Ca2+ imaging and pharmacological manipulation of the sodium calcium exchanger. These experiments suggest that the sodium calcium exchanger may be switching to a reverse-mode configuration in the CGCs during aging.
Original languageEnglish
Pages (from-to)2838-2849
Number of pages12
JournalNeurobiology of Aging
Volume36
Issue number10
DOIs
Publication statusPublished - 1 Oct 2015

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Sodium-Calcium Exchanger
Giant Cells
Cell Aging
Lymnaea
Snails
Pharmacology
Neurons

Bibliographical note

© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • Sodium calcium exchanger
  • NCX
  • Lymnaea stagnalis
  • Aging
  • Slow afterhyperpolarization
  • Cerebral giant cells

Cite this

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title = "A switch in the mode of the sodium/calcium exchanger underlies an age-related increase in the slow afterhyperpolarization",
abstract = "During aging, the Ca2+-sensitive slow afterhyperpolarization (sAHP) of hippocampal neurons is known to increase in duration. This change has also been observed in the serotonergic cerebral giant cells (CGCs) of the pond snail Lymnaea stagnalis, but has yet to be characterized. In this article, we confirm that there is a reduction in firing rate, an increase in the duration of the sAHP, and an alteration in the strength and speed of spike frequency adaptation in the CGCs during aging, a finding that is compatible with an increase in the sAHP current. We go on to show that age-related changes in the kinetics of spike frequency adaptation are consistent with a reduction in Ca2+ clearance from the cell, which we confirm with Ca2+ imaging and pharmacological manipulation of the sodium calcium exchanger. These experiments suggest that the sodium calcium exchanger may be switching to a reverse-mode configuration in the CGCs during aging.",
keywords = "Sodium calcium exchanger, NCX, Lymnaea stagnalis, Aging, Slow afterhyperpolarization, Cerebral giant cells",
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A switch in the mode of the sodium/calcium exchanger underlies an age-related increase in the slow afterhyperpolarization. / Scutt, Greg; Allen, Marcus; Kemenes, György; Yeoman, Mark.

In: Neurobiology of Aging, Vol. 36, No. 10, 01.10.2015, p. 2838-2849.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A switch in the mode of the sodium/calcium exchanger underlies an age-related increase in the slow afterhyperpolarization

AU - Scutt, Greg

AU - Allen, Marcus

AU - Kemenes, György

AU - Yeoman, Mark

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