A serum-free and feeder-free method for expanding human keratinocytes on microcarriers for the treatment of severe burn injuries

Y.H. Martin, Anthony Metcalfe

Research output: Contribution to journalArticle

Abstract

Autologous keratinocytes are used in the treatment of severe burns to augment wound healing. Cells are commonly expanded in serum-containing medium in the presence of lethally irradiated mouse fibroblast feeder cells. Application to the wound bed in single cell suspension is damaging to the cells and often results in significant cell loss. We previously demonstrated improved wound healing outcomes in the porcine model of wound repair with cells delivered using biodegradable gelatin microcarriers instead of as cell spray1. We present here an improved method of culturing human keratinocytes on biodegradable microcarriers under serum-free and feeder-free conditions.
Original languageEnglish
JournalWound Repair and Regeneration
Volume23
Issue number4
Publication statusPublished - 31 Aug 2015

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Keratinocytes
Wounds and Injuries
Serum
Wound Healing
Feeder Cells
Gelatin
Burns
Suspensions
Swine
Fibroblasts

Bibliographical note

This is the peer reviewed version of the following article: Martin Y., Metcalfe T. (2015) A serum-free and feeder-free method for expanding human keratinocytes on microcarriers for the treatment of severe burn injuries, Wound Repair and Regeneration, Volume 23, Issue 4, pages A1–A37, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/wrr.12342/abstract. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Cite this

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abstract = "Autologous keratinocytes are used in the treatment of severe burns to augment wound healing. Cells are commonly expanded in serum-containing medium in the presence of lethally irradiated mouse fibroblast feeder cells. Application to the wound bed in single cell suspension is damaging to the cells and often results in significant cell loss. We previously demonstrated improved wound healing outcomes in the porcine model of wound repair with cells delivered using biodegradable gelatin microcarriers instead of as cell spray1. We present here an improved method of culturing human keratinocytes on biodegradable microcarriers under serum-free and feeder-free conditions.",
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A serum-free and feeder-free method for expanding human keratinocytes on microcarriers for the treatment of severe burn injuries. / Martin, Y.H.; Metcalfe, Anthony.

In: Wound Repair and Regeneration, Vol. 23, No. 4, 31.08.2015.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Martin, Y.H.

AU - Metcalfe, Anthony

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N2 - Autologous keratinocytes are used in the treatment of severe burns to augment wound healing. Cells are commonly expanded in serum-containing medium in the presence of lethally irradiated mouse fibroblast feeder cells. Application to the wound bed in single cell suspension is damaging to the cells and often results in significant cell loss. We previously demonstrated improved wound healing outcomes in the porcine model of wound repair with cells delivered using biodegradable gelatin microcarriers instead of as cell spray1. We present here an improved method of culturing human keratinocytes on biodegradable microcarriers under serum-free and feeder-free conditions.

AB - Autologous keratinocytes are used in the treatment of severe burns to augment wound healing. Cells are commonly expanded in serum-containing medium in the presence of lethally irradiated mouse fibroblast feeder cells. Application to the wound bed in single cell suspension is damaging to the cells and often results in significant cell loss. We previously demonstrated improved wound healing outcomes in the porcine model of wound repair with cells delivered using biodegradable gelatin microcarriers instead of as cell spray1. We present here an improved method of culturing human keratinocytes on biodegradable microcarriers under serum-free and feeder-free conditions.

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JO - Wound Repair and Regeneration

JF - Wound Repair and Regeneration

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