A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam

Elizabeth Ostler; C.J. Dean; N. Barber; M. Valeri; Stuart James; M. Resmini; G. Boucher; N. Romanov; K. Brocklehurst; Gerard Gallacher

doi:10.1016/S0006-291X(02)02604-9

A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam

Elizabeth Ostler
, C.J. Dean
, N. Barber
, M. Valeri
, Stuart James
, M. Resmini
, G. Boucher
, N. Romanov
, K. Brocklehurst
, Gerard Gallacher

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

We report the first example of a monoclonal antibody-catalysed hydrolysis of a β-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with kcat=8.7×10−6 s−1 and KM=35 μM. This is the first example of a rat monoclonal catalytic antibody.

Original language	English
Pages (from-to)	273-276
Number of pages	4
Journal	Biochemical and biophysical research communications
Volume	299
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(02)02604-9
Publication status	Published - Nov 2002

Keywords

Catalytic antibodies
β-Lactam
Hydrolysis
Monoclonal antibodies
Abzyme

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title = "A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam",

abstract = "We report the first example of a monoclonal antibody-catalysed hydrolysis of a β-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with kcat=8.7×10−6 s−1 and KM=35 μM. This is the first example of a rat monoclonal catalytic antibody.",

keywords = "Catalytic antibodies, β-Lactam, Hydrolysis, Monoclonal antibodies, Abzyme",

author = "Elizabeth Ostler and C.J. Dean and N. Barber and M. Valeri and Stuart James and M. Resmini and G. Boucher and N. Romanov and K. Brocklehurst and Gerard Gallacher",

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T1 - A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam

AU - Ostler, Elizabeth

AU - Dean, C.J.

AU - Barber, N.

AU - Valeri, M.

AU - James, Stuart

AU - Resmini, M.

AU - Boucher, G.

AU - Romanov, N.

AU - Brocklehurst, K.

AU - Gallacher, Gerard

PY - 2002/11

Y1 - 2002/11

N2 - We report the first example of a monoclonal antibody-catalysed hydrolysis of a β-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with kcat=8.7×10−6 s−1 and KM=35 μM. This is the first example of a rat monoclonal catalytic antibody.

AB - We report the first example of a monoclonal antibody-catalysed hydrolysis of a β-lactam where the antibodies were generated by a simple transition-state analogue. A rat monoclonal antibody (1/91c/4d/26) generated by using an acyclic 4-nitrophenylphosphate immunogen catalysed the hydrolysis of corresponding 4-nitrophenyl carbonates but, more importantly, also catalysed the hydrolysis of N-(4-nitrophenyl)-azetidinone at pH 8 with kcat=8.7×10−6 s−1 and KM=35 μM. This is the first example of a rat monoclonal catalytic antibody.

KW - Catalytic antibodies

KW - β-Lactam

KW - Hydrolysis

KW - Monoclonal antibodies

KW - Abzyme

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DO - 10.1016/S0006-291X(02)02604-9

M3 - Article

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SP - 273

EP - 276

JO - Biochemical and biophysical research communications

JF - Biochemical and biophysical research communications

IS - 2

ER -

A rat monoclonal antibody that catalyses the hydrolysis of a nitrophenyl-β-lactam

Abstract

Keywords

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