A novel carbon device for the treatment of sepsis

C. A. Howell; S. R. Sandeman; G. J. Phillips; A. W. Lloyd; S. V. Mikhalovsky; J. G. Davies; S. R. Tennison; O. Kozynchemko; J. D.S. Gaylor; J. M. Courtney

A novel carbon device for the treatment of sepsis

C. A. Howell
, S. R. Sandeman
, G. J. Phillips
, A. W. Lloyd
, S. V. Mikhalovsky
, J. G. Davies
, S. R. Tennison
, O. Kozynchemko
, J. D.S. Gaylor
, J. M. Courtney

Research output: Chapter in Book/Conference proceeding with ISSN or ISBN › Conference contribution with ISSN or ISBN › peer-review

Abstract

The potential of carbon dics to remove the pre-inflammatory cytokine TNF-α from culture medium harvested from lipopolysaccharides (LPS) stimulated cells was investigated. Raw 264.7 cells were cultured in Dulbecco Modified Eagle' medium (DMEM) containing 10% v/v foetal calf serum (FCS). Cytokine containing media and control media were harvested at 2, 4, 6, 8, 24, and 28 hours, and centrifuged at 1500rpm for 5 minutes prior to analysis of the supplement for TNF-α concentration. The efficient removal of TNF-α by the discs suggest that a combined adsorption/filtration device produced from carbon may provide a new method of removing inflammatory mediators from the circulation of septic patients.

Original language	English
Title of host publication	Transactions - 7th World Biomaterials Congress
Pages	559
Number of pages	1
Publication status	Published - 2004
Event	Transactions - 7th World Biomaterials Congress - Sydney, Australia Duration: 17 May 2004 → 21 May 2004

Publication series

Name	Transactions - 7th World Biomaterials Congress

Conference

Conference	Transactions - 7th World Biomaterials Congress
Country/Territory	Australia
City	Sydney
Period	17/05/04 → 21/05/04

Cite this

@inproceedings{8e66ab3c70aa4bf582b97933fbc6642a,

title = "A novel carbon device for the treatment of sepsis",

abstract = "The potential of carbon dics to remove the pre-inflammatory cytokine TNF-α from culture medium harvested from lipopolysaccharides (LPS) stimulated cells was investigated. Raw 264.7 cells were cultured in Dulbecco Modified Eagle' medium (DMEM) containing 10\% v/v foetal calf serum (FCS). Cytokine containing media and control media were harvested at 2, 4, 6, 8, 24, and 28 hours, and centrifuged at 1500rpm for 5 minutes prior to analysis of the supplement for TNF-α concentration. The efficient removal of TNF-α by the discs suggest that a combined adsorption/filtration device produced from carbon may provide a new method of removing inflammatory mediators from the circulation of septic patients.",

author = "Howell, \{C. A.\} and Sandeman, \{S. R.\} and Phillips, \{G. J.\} and Lloyd, \{A. W.\} and Mikhalovsky, \{S. V.\} and Davies, \{J. G.\} and Tennison, \{S. R.\} and O. Kozynchemko and Gaylor, \{J. D.S.\} and Courtney, \{J. M.\}",

year = "2004",

language = "English",

isbn = "1877040193",

series = "Transactions - 7th World Biomaterials Congress",

pages = "559",

booktitle = "Transactions - 7th World Biomaterials Congress",

note = "Transactions - 7th World Biomaterials Congress ; Conference date: 17-05-2004 Through 21-05-2004",

}

Howell, CA, Sandeman, SR, Phillips, GJ, Lloyd, AW, Mikhalovsky, SV, Davies, JG, Tennison, SR, Kozynchemko, O, Gaylor, JDS & Courtney, JM 2004, A novel carbon device for the treatment of sepsis. in Transactions - 7th World Biomaterials Congress. Transactions - 7th World Biomaterials Congress, pp. 559, Transactions - 7th World Biomaterials Congress, Sydney, Australia, 17/05/04.

TY - GEN

T1 - A novel carbon device for the treatment of sepsis

AU - Howell, C. A.

AU - Sandeman, S. R.

AU - Phillips, G. J.

AU - Lloyd, A. W.

AU - Mikhalovsky, S. V.

AU - Davies, J. G.

AU - Tennison, S. R.

AU - Kozynchemko, O.

AU - Gaylor, J. D.S.

AU - Courtney, J. M.

PY - 2004

Y1 - 2004

N2 - The potential of carbon dics to remove the pre-inflammatory cytokine TNF-α from culture medium harvested from lipopolysaccharides (LPS) stimulated cells was investigated. Raw 264.7 cells were cultured in Dulbecco Modified Eagle' medium (DMEM) containing 10% v/v foetal calf serum (FCS). Cytokine containing media and control media were harvested at 2, 4, 6, 8, 24, and 28 hours, and centrifuged at 1500rpm for 5 minutes prior to analysis of the supplement for TNF-α concentration. The efficient removal of TNF-α by the discs suggest that a combined adsorption/filtration device produced from carbon may provide a new method of removing inflammatory mediators from the circulation of septic patients.

AB - The potential of carbon dics to remove the pre-inflammatory cytokine TNF-α from culture medium harvested from lipopolysaccharides (LPS) stimulated cells was investigated. Raw 264.7 cells were cultured in Dulbecco Modified Eagle' medium (DMEM) containing 10% v/v foetal calf serum (FCS). Cytokine containing media and control media were harvested at 2, 4, 6, 8, 24, and 28 hours, and centrifuged at 1500rpm for 5 minutes prior to analysis of the supplement for TNF-α concentration. The efficient removal of TNF-α by the discs suggest that a combined adsorption/filtration device produced from carbon may provide a new method of removing inflammatory mediators from the circulation of septic patients.

UR - https://www.scopus.com/pages/publications/13844255653

M3 - Conference contribution with ISSN or ISBN

AN - SCOPUS:13844255653

SN - 1877040193

SN - 9781877040191

T3 - Transactions - 7th World Biomaterials Congress

SP - 559

BT - Transactions - 7th World Biomaterials Congress

T2 - Transactions - 7th World Biomaterials Congress

Y2 - 17 May 2004 through 21 May 2004

ER -

A novel carbon device for the treatment of sepsis

Abstract

Publication series

Conference

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