A novel carbon device for the treatment of sepsis

C. A. Howell, S. R. Sandeman, G. J. Phillips, A. W. Lloyd, S. V. Mikhalovsky, J. G. Davies, S. R. Tennison, O. Kozynchemko, J. D.S. Gaylor, J. M. Courtney

Research output: Chapter in Book/Conference proceeding with ISSN or ISBNConference contribution with ISSN or ISBNpeer-review


The potential of carbon dics to remove the pre-inflammatory cytokine TNF-α from culture medium harvested from lipopolysaccharides (LPS) stimulated cells was investigated. Raw 264.7 cells were cultured in Dulbecco Modified Eagle' medium (DMEM) containing 10% v/v foetal calf serum (FCS). Cytokine containing media and control media were harvested at 2, 4, 6, 8, 24, and 28 hours, and centrifuged at 1500rpm for 5 minutes prior to analysis of the supplement for TNF-α concentration. The efficient removal of TNF-α by the discs suggest that a combined adsorption/filtration device produced from carbon may provide a new method of removing inflammatory mediators from the circulation of septic patients.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 2004
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 17 May 200421 May 2004

Publication series

NameTransactions - 7th World Biomaterials Congress


ConferenceTransactions - 7th World Biomaterials Congress


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