A galactokinase-like protein from the liver fluke Fasciola hepatica

Veronika L. Zinsser, Ciara Cox, Margaret McAuley, Elizabeth M. Hoey, Alan Trudgett, David J. Timson

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Galactokinase catalyses the ATP-dependent phosphorylation of galactose. A galactokinase-like sequence was identified in a Fasciola hepatica EST library. Recombinant expression of the corresponding protein in Escherichia coli resulted in a protein of approximately 50 kDa. The protein is monomeric, like galactokinases from higher animals, yeasts and some bacteria. The protein has no detectable enzymatic activity with galactose or N-acetylgalactosamine as a substrate. However, it does bind to ATP. Molecular modelling predicted that the protein adopts a similar fold to galactokinase and other GHMP kinases. However, a key loop in the active site was identified which may influence the lack of activity. Sequence analysis strongly suggested that this protein (and other proteins annotated as “galactokinase” in the trematodes Schistosoma mansoni and Clonorchis sinensis) are closer to N-acetylgalactosamine kinases. No other galactokinase-like sequences appear to be present in the genomes of these three species. This raises the intriguing possibility that these (and possibly other) trematodes are unable to catabolise galactose through the Leloir pathway due to the lack of a functional galactokinase.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalExperimental Parasitology
Volume192
DOIs
Publication statusPublished - 21 Jul 2018

Fingerprint

Galactokinase
Fasciola hepatica
Galactose
Acetylgalactosamine
Proteins
Phosphotransferases
Adenosine Triphosphate
Clonorchis sinensis
Schistosoma mansoni
Escherichia coli Proteins
Expressed Sequence Tags
Sequence Analysis
Catalytic Domain
Yeasts
Phosphorylation
Genome
Bacteria

Keywords

  • Fascioliasis
  • Galactokinase
  • GHMP kinase
  • Leloir pathway
  • N-acetylgalactosamine
  • Trematode metabolism

Cite this

Zinsser, Veronika L. ; Cox, Ciara ; McAuley, Margaret ; Hoey, Elizabeth M. ; Trudgett, Alan ; Timson, David J. / A galactokinase-like protein from the liver fluke Fasciola hepatica. In: Experimental Parasitology. 2018 ; Vol. 192. pp. 65-72.
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A galactokinase-like protein from the liver fluke Fasciola hepatica. / Zinsser, Veronika L.; Cox, Ciara; McAuley, Margaret; Hoey, Elizabeth M.; Trudgett, Alan; Timson, David J.

In: Experimental Parasitology, Vol. 192, 21.07.2018, p. 65-72.

Research output: Contribution to journalArticleResearchpeer-review

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