A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion

S. Kockisch, G.D. Rees, S.A. Young, J. Tsibouklis, John Smart

Research output: Contribution to journalArticle

Abstract

A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.
Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalJournal of Controlled Release
Volume77
Issue number1-2
DOIs
Publication statusPublished - Nov 2001

Fingerprint

Polymers
Adhesion
Mouthwashes
Alcian Blue
Chitosan
Eosine Yellowish-(YS)
Dispersions
Washing
Image analysis
Sucrose
Coloring Agents
Cells

Keywords

  • Bioadhesion
  • Oral cavity
  • Poly(acrylic acid)
  • Chitosan

Cite this

Kockisch, S. ; Rees, G.D. ; Young, S.A. ; Tsibouklis, J. ; Smart, John. / A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion. In: Journal of Controlled Release. 2001 ; Vol. 77, No. 1-2. pp. 1-6.
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A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion. / Kockisch, S.; Rees, G.D.; Young, S.A.; Tsibouklis, J.; Smart, John.

In: Journal of Controlled Release, Vol. 77, No. 1-2, 11.2001, p. 1-6.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A direct-staining method to evaluate the mucoadhesion of polymers from aqueous dispersion

AU - Kockisch, S.

AU - Rees, G.D.

AU - Young, S.A.

AU - Tsibouklis, J.

AU - Smart, John

PY - 2001/11

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AB - A novel technique to evaluate polymer adhesion to human buccal cells following exposure to aqueous polymer dispersion, both in vitro and in vivo, is described. Adhering polymer has been visualised by staining with 0.1% (w/v) of either Alcian blue (60 min) or Eosin (10 min) solution, uncomplexed dye being removed by 0.25 M sucrose washings. The extent of polymer adhesion was quantified by measuring the relative staining intensity of control and polymer-treated cells by image analysis. In vitro, Carbopol 974P, polycarbophil (Noveon AA-1) and chitosan (CL 113) were found to adhere to human buccal cells from 0.10% (w/w) aqueous dispersions of these polymers. Following in vivo administration as a mouthwash, these polymers persisted upon the human buccal mucosa for at least 1 h.

KW - Bioadhesion

KW - Oral cavity

KW - Poly(acrylic acid)

KW - Chitosan

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DO - 10.1016/S0168-3659(01)00444-8

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JF - Journal of Controlled Release

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