TY - JOUR
T1 - A de novo virus-like topology for synthetic virions
AU - Noble, James
AU - De Santis, Emiliana
AU - Ravi, Jascindra
AU - Castelletto, Valeria
AU - Lamarre, Baptiste
AU - Mantell, Judith
AU - Ray, Santanu
AU - Ryadnov, Maxim G.
N1 - © 2016 American Chemical Society
PY - 2016/9/1
Y1 - 2016/9/1
N2 - A de novo topology of virus-like assembly is reported. The design is a trifaceted coiled-coil peptide helix, which self-assembles into ultrasmall, monodisperse, anionic virus-like shells that encapsulate and transfer both RNA and DNA into human cells. Unlike existing artificial systems, these shells share the same physical characteristics of viruses being anionic, nonaggregating, abundant, hollow, and uniform in size, while effectively mediating gene silencing and transgene expression. These are the smallest virus-like structures reported to date, both synthetic and native, with the ability to adapt and transfer small and large nucleic acids. The design thus offers a promising solution for engineering bespoke artificial viruses with desired functions.
AB - A de novo topology of virus-like assembly is reported. The design is a trifaceted coiled-coil peptide helix, which self-assembles into ultrasmall, monodisperse, anionic virus-like shells that encapsulate and transfer both RNA and DNA into human cells. Unlike existing artificial systems, these shells share the same physical characteristics of viruses being anionic, nonaggregating, abundant, hollow, and uniform in size, while effectively mediating gene silencing and transgene expression. These are the smallest virus-like structures reported to date, both synthetic and native, with the ability to adapt and transfer small and large nucleic acids. The design thus offers a promising solution for engineering bespoke artificial viruses with desired functions.
U2 - 10.1021/jacs.6b05751
DO - 10.1021/jacs.6b05751
M3 - Article
SN - 0002-7863
VL - 138
SP - 12202
EP - 12210
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 37
ER -