A ﬁeld evaluation of an isothermal DNA ampliﬁcation assay for the detection of Theileria annulata infection in cattle

Jacinto Gomes; Marcos Santos; Ana Amaro; Alvaro Fonseca; Gabriela Santos-Gomes; Joao Inacio Silva

doi:10.1016/j.mcp.2016.12.006

A ﬁeld evaluation of an isothermal DNA ampliﬁcation assay for the detection of Theileria annulata infection in cattle

Jacinto Gomes, Marcos Santos, Ana Amaro, Alvaro Fonseca, Gabriela Santos-Gomes, Joao Inacio Silva

University of Brighton

Research output: Contribution to journal › Article › peer-review

Abstract

A loop-mediated isothermal ampliﬁcation (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantiﬁcation of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respec- tively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating ahigh analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.

Original language	English
Pages (from-to)	61-64
Number of pages	4
Journal	Molecular and Cellular Probes
Volume	31
DOIs	https://doi.org/10.1016/j.mcp.2016.12.006
Publication status	Published - 22 Dec 2016

Bibliographical note

Keywords

Molecular diagnostics
LAMP
Real-time PCR
Tams1
Theileria annulata
Bovine theileriosis

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Gomes 2016 Theileria annulata LAMP cattle Revision2.pdfAccepted author manuscript, 104 KBLicence: CC BY-NC-ND

Joao Inacio Silva
- J.Inaciobrighton.acuk
- School of Applied Sciences - Principal Lecturer
- Ecology, Conservation and Society Research Excellence Group
- Centre for Lifelong Health
Person: Academic

Cite this

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title = "A ﬁeld evaluation of an isothermal DNA ampliﬁcation assay for the detection of Theileria annulata infection in cattle",

abstract = "A loop-mediated isothermal ampliﬁcation (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantiﬁcation of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respec- tively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating ahigh analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.",

keywords = "Molecular diagnostics, LAMP, Real-time PCR, Tams1, Theileria annulata, Bovine theileriosis",

author = "Jacinto Gomes and Marcos Santos and Ana Amaro and Alvaro Fonseca and Gabriela Santos-Gomes and {Inacio Silva}, Joao",

note = "{\textcopyright} 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/",

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T1 - A ﬁeld evaluation of an isothermal DNA ampliﬁcation assay for the detection of Theileria annulata infection in cattle

AU - Gomes, Jacinto

AU - Santos, Marcos

AU - Amaro, Ana

AU - Fonseca, Alvaro

AU - Santos-Gomes, Gabriela

AU - Inacio Silva, Joao

PY - 2016/12/22

Y1 - 2016/12/22

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AB - A loop-mediated isothermal ampliﬁcation (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantiﬁcation of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respec- tively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating ahigh analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.

KW - Molecular diagnostics

KW - LAMP

KW - Real-time PCR

KW - Tams1

KW - Theileria annulata

KW - Bovine theileriosis

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DO - 10.1016/j.mcp.2016.12.006

M3 - Article

SN - 0890-8508

VL - 31

SP - 61

EP - 64

JO - Molecular and Cellular Probes

JF - Molecular and Cellular Probes

ER -

A ﬁeld evaluation of an isothermal DNA ampliﬁcation assay for the detection of Theileria annulata infection in cattle

Abstract

Bibliographical note

Keywords

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Joao Inacio Silva

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