A loop-mediated isothermal ampliﬁcation (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantiﬁcation of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respec- tively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating ahigh analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.
Bibliographical note© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Molecular diagnostics
- Real-time PCR
- Theileria annulata
- Bovine theileriosis
Gomes, J., Santos, M., Amaro, A., Fonseca, A., Santos-Gomes, G., & Inacio Silva, J. (2016). A ﬁeld evaluation of an isothermal DNA ampliﬁcation assay for the detection of Theileria annulata infection in cattle. Molecular and Cellular Probes, 31, 61-64. https://doi.org/10.1016/j.mcp.2016.12.006