A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle

Jacinto Gomes, Marcos Santos, Ana Amaro, Alvaro Fonseca, Gabriela Santos-Gomes, Joao Inacio Silva

Research output: Contribution to journalArticle

Abstract

A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respec- tively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating ahigh analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.
Original languageEnglish
Pages (from-to)61-64
Number of pages4
JournalMolecular and Cellular Probes
Volume31
DOIs
Publication statusPublished - 22 Dec 2016

Bibliographical note

© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • Molecular diagnostics
  • LAMP
  • Real-time PCR
  • Tams1
  • Theileria annulata
  • Bovine theileriosis

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