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A Dynamic Core in Human NQO1 Controls the Functional and Stability Effects of Ligand Binding and Their Communication across the Enzyme Dimer

Vankova, P., Salido, E., Timson, D., Man, P. & Pey, A. L., 12 Nov 2019, In : Biomolecules. 9, 11, 728.

Research output: Contribution to journalArticle

Open Access
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Flavin-Adenine Dinucleotide
Dicumarol
Dimers
Ligands
Communication

A galactokinase-like protein from the liver fluke Fasciola hepatica

Zinsser, V. L., Cox, C., McAuley, M., Hoey, E. M., Trudgett, A. & Timson, D. J., 21 Jul 2018, In : Experimental Parasitology. 192, p. 65-72 8 p.

Research output: Contribution to journalArticle

Open Access
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Galactokinase
Fasciola hepatica
Galactose
Acetylgalactosamine
Proteins

A ligand predication tool based on modeling and reasoning with imprecise probabilistic knowledge

Liu, W., Yue, A. & Timson, D., 30 Apr 2010, In : Computer Methods and Programs in Biomedicine. 98, 1, p. 45-54 10 p.

Research output: Contribution to journalArticle

A mysterious family of calcium-binding proteins from parasitic worms

Thomas, C. M. & Timson, D. J., 15 Aug 2016, In : Biochemical Society Transactions. 44, 4, p. 1005-1010 6 p.

Research output: Contribution to journalArticle

Open Access
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Calcium-Binding Proteins
Helminths
Ions
Calcium
EF Hand Motifs

Antioxidant properties and global metabolite screening ofthe probiotic yeast Saccharomyces cerevisiae var. boulardii

Datta, S., Timson, D. & Annapure, U. S., 21 Nov 2016, In : Journal of the Science of Food and Agriculture. 97, 9, p. 3039-3049 11 p.

Research output: Contribution to journalArticle

Open Access
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probiotics
Saccharomyces cerevisiae
metabolites
yeasts
screening

Biochemical analysis of the interactions of IQGAP1 C-terminal domain with CDC42

Elliott, S. F., Allen, G. & Timson, D., 26 Mar 2012, In : World Journal of Biological Chemistry. 3, 3, p. 53-60

Research output: Contribution to journalArticle

Open Access
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Proteins
Neoplasms
Mutation
Ethyldimethylaminopropyl Carbodiimide
Phosphorylation

Biochemical characterisation of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from the liver fluke, Fasciola hepatica

Zinsser, V. L., Hoey, E. M., Trudgett, A. & Timson, D., 30 Apr 2014, In : BBA - Proteins and Proteomics. 1844, 4, p. 744-749 6 p.

Research output: Contribution to journalArticle

Calmodulin disruption impacts growth and motility in juvenile liver fluke

Timson, D., 27 Jan 2016, In : Parasites & Vectors. 9, p. 1-13 13 p.

Research output: Contribution to journalArticle

Open Access
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Fasciola hepatica
Calmodulin
Growth
Calcium
Fasciola
Open Access
File
Schistosoma mansoni
Calmodulin
Calcium Channels
Calcium
Ions

Cancer-associated variants of human NQO1: Impacts on inhibitor binding and cooperativity

Megarity, C. F. & Timson, D. J., 6 Sep 2019, In : Bioscience Reports. 39, 9, BSR20191874.

Research output: Contribution to journalArticle

Open Access
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Dicumarol
Niacinamide
Oxidoreductases
Quinine
Enzymes

Catalytic mechanism of mevalonate kinase revisited, a QM/MM study

McClory, J., Lin, J. T., Timson, D., Zhang, J. & Huang, M., 5 Feb 2019, In : Organic and Biomolecular Chemistry. 17, 9, p. 2423-2431 9 p.

Research output: Contribution to journalArticle

Open Access
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mevalonate kinase
Mevalonic Acid
Adenosine Triphosphate
Biofuels
Phosphorylation

Cause or effect: which genetic changes are associated with cancer?

Timson, D., 2014, In : Gene, Cell and Tissue. 1, 3, e23818.

Research output: Contribution to journalArticle

Open Access
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Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms

Cray, J. A., Stevenson, A., Ball, P., Bankar, S. B., Eleutherio, E. C. A., Ezeji, T. C., Singhal, R. S., Thevelein, J. M., Timson, D. & Hallsworth, J. E., 30 Jun 2015, In : Current Opinion in Biotechnology. 33, p. 228-259 32 p.

Research output: Contribution to journalArticle

Characterisation of eppin function: expression and activity in the lung

Scott, A., Glasgow, A., Small, D., Carlile, S., McCrudden, M. T. C., McLean, D. T. F., Brown, R., Doherty, D., Lundy, F. T., Hamid, U. I., O'Kane, C. M., McAuley, D. F., Brodlie, M., Tunney, M., Elborn, J. S., Irwin, C. R., Timson, D., Taggart, C. C. & Weldon, S., 13 Jul 2017, In : European Respiratory Journal. 50, 1

Research output: Contribution to journalArticle

Open Access
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Lipopolysaccharides
Myeloid Cells
Lung
Chemokine CXCL2
Serine Proteinase Inhibitors

Characterization of Cd36_03230p, a putative vanillin dehydrogenase from Candida dubliniensis

Datta, S., Annapure, U. S. & Timson, D., 14 Oct 2016, In : RSC Advances. 6, 102, p. 99774-99780 7 p.

Research output: Contribution to journalArticle

Open Access
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Candida
Aldehyde Dehydrogenase
Substrates
Yeast
CD36 Antigens

Comparative biochemical analysis of three members of the Schistosoma mansoni TAL family: Differences in ion and drug binding properties

Thomas, C. M., Fitzsimmons, C. M., Timson, D. & Dunne, D. W., 31 Jan 2015, In : Biochimie. 108, p. 40-47 8 p.

Research output: Contribution to journalArticle

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Conformational dynamics is key to understanding loss-of-function of NQO1 cancer-associated polymorphisms and its correction by pharmacological ligands

Medina-Carmona, E., Palomino-Morales, R. J., Fuchs, J. E., Padin-Gonzalez, E., Mesa-Torres, N., Salido, E., Timson, D. & Pey, A. L., 3 Feb 2016, In : Scientific Reports. 6

Research output: Contribution to journalArticle

Open Access
File
Flavin-Adenine Dinucleotide
Pharmacology
Ligands
Neoplasms
Dicumarol

Dicoumarol: a drug which hits at least two very different targets in vitamin K metabolism

Timson, D., 22 Jul 2015, In : Current Drug Targets. 16, p. 1-11 11 p.

Research output: Contribution to journalArticle

Open Access
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Dicumarol
Vitamin K
Warfarin
Vitamin K Epoxide Reductases
Pharmaceutical Preparations

Different specificities of two aldehyde dehydrogenases from Saccharomyces cerevisiae var boulardii

Datta, S., Annapore, U. S. & Timson, D., 26 Jan 2017, In : Bioscience Reports. 37, 2

Research output: Contribution to journalArticle

Open Access
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aldehyde dehydrogenase
aldehydes
Saccharomyces cerevisiae
active sites
isozymes

Disturbed cofactor binding by a novel mutation in UDP-galactose 4 '-epimerase results in a type III galactosemia phenotype at birth

Paul, S., McCorvie, T. J., Zschocke, J. & Timson, D., 3 Feb 2016, In : RSC Advances. 6, 21, p. 17297-17301 5 p.

Research output: Contribution to journalArticle

Open Access
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UDPglucose 4-Epimerase
Galactosemias
Galactose
Parturition
Phenotype

Dynamic origins of substrate promiscuity in bacterial galactokinases

McAuley, M., Huang, M. & Timson, D., 17 Oct 2019, In : Carbohydrate Research. 486, 107839.

Research output: Contribution to journalArticle

Enzymes
Escherichia coli
Galactokinase
Sugar Phosphates
Bacterial Structures

Enhanced vulnerability of human proteins towards disease-associated inactivation through divergent evolution

Medina-Carmona, E., Fuchs, J. E., Gavira, J. A., Mesa-Torres, N., Neira, J. L., Salido, E., Palomino-Morales, R. J., Burgos, M., Timson, D. & Pey, A. L., 6 Jul 2017, In : Human Molecular Genetics. 26, 18, p. 3531-3544 14 p.

Research output: Contribution to journalArticle

Open Access
File
Proteins
Amino Acids
Mutation
Protein Stability
Genetic Suppression
Dicumarol
Eukaryota
Oxidoreductases
Escherichia coli
Alanine

Evidence for chaotropicity/kosmotropicity offset in a yeast growth model

Eardley, J., Dedi, C., Dymond, M., Hallsworth, J. E. & Timson, D., 26 Sep 2019, In : Biotechnology Letters. 41, 11, p. 1309-1318 10 p.

Research output: Contribution to journalArticle

yeast
ethanol
ammonium sulfate
fermentation
solute

Experimental and computational evidence on conformational fluctuations as a source of catalytic defects in genetic diseases

Fuchs, J. E., Munoz, I. G., Timson, D. & Pey, A. L., 13 Jun 2016, In : RSC Advances. 6, 63, p. 58604-58612 9 p.

Research output: Contribution to journalArticle

Open Access
File
NAD
Defects
Molecular dynamics
UDPglucose 4-Epimerase
Thermodynamics

FAD binding overcomes defects in activity and stability displayed by cancer-associated variants of human NQO1

Pey, A. L., Megarity, C. F. & Timson, D., 30 Nov 2014, In : BBA - Molecular Basis of Disease. 1842, 11, p. 2163-2173 11 p.

Research output: Contribution to journalArticle

Fasciola hepaticacalcium binding protein FhCaBP2: Structure of the dynein light chain-like domain

Nguyen, T. H., Thomas, C. M., Timson, D. & Van Raaij, M. J., 16 Apr 2016, In : Parasitology Research. 115, 7, p. 2879-2886 8 p.

Research output: Contribution to journalArticle

Open Access
File
Fasciola
Dyneins
Fasciola hepatica
Carrier Proteins
EF Hand Motifs

FhCaBP1 (FH22): a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

Thomas, C. M., Cheung, S. & Timson, D., 28 Sep 2016, In : Experimental Parasitology. 170, p. 109-115 7 p.

Research output: Contribution to journalArticle

Open Access
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Dyneins
Calcium-Binding Proteins
Ions
Calcium
Proteins

FhCaBP2: a Fasciola hepatica calcium-binding protein with EF-hand and dynein light chain domains

Thomas, C. M. & Timson, D., 30 Sep 2015, In : Parasitology. 142, 11, p. 1375-1386 12 p.

Research output: Contribution to journalArticle

Four Challenges for Better Biocatalysts

Timson, D. J., 9 May 2019, In : Fermentation. 5, 2, 9 p., 39.

Research output: Contribution to journalArticle

Open Access
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Enzymes
Protein folding
Biochemistry
Molecules
Biomolecules

Fructose 1,6-bisphosphatase: getting the message across

Timson, D., 6 Mar 2019, In : Bioscience Reports. 39, 3, p. 1-5 5 p., BSR20190124.

Research output: Contribution to journalArticle

Open Access
File
Fructose-Bisphosphatase
Adenosine Monophosphate
Enzymes
Binding Sites
Allosteric Regulation

Galactokinase promiscuity: A question of flexibility?

McAuley, M., Kristiansson, H., Huang, M., Pey, A. L. & Timson, D. J., 9 Feb 2016, In : Biochemical Society Transactions. 44, p. 116-122 7 p.

Research output: Contribution to journalArticle

Open Access
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Galactokinase
Galactosemias
Sugars
Catalytic Domain
Enzymes

Galactokinases: Potential Biotechnological Applications as Biocatalysts

Kristiansson, H. & Timson, D., 2012, In : Current Biotechnology. 1, 2, p. 148-154 7 p.

Research output: Contribution to journalArticle

Enzymes
Galactokinase
Galactose
Phosphotransferases
homoserine kinase

Glycerol enhances fungal germination at the water-activity limit for life

Stevenson, A., Hamill, P. G., Medina, A., Kminek, G., Rummel, J. D., Dijksterhuis, J., Timson, D., Magan, N., Leong, S. L. & Hallsworth, J. E., 13 Nov 2016, In : Environmental Microbiology. 19, p. 947-967 21 p.

Research output: Contribution to journalArticle

Open Access
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water activity
glycerol
germination
xerophytes
spores

Improving the activity and stability of human galactokinase for therapeutic and biotechnological applications

McAuley, M., Mesa-Torres, N., McFall, A., Morris, S., Huang, M., Pey, A. L. & Timson, D., 26 Apr 2018, In : ChemBioChem. 19, 10, p. 1088-1095 8 p.

Research output: Contribution to journalArticle

Open Access
File
Galactokinase
Human Activities
Enzyme Replacement Therapy
Enzymes
Galactosemias

Insight into the mechanism of galactokinase: role of a critical glutamate residue and helix/coil transitions

McAuley, M., Huang, M. & Timson, D., 24 Oct 2016, In : BBA - Proteins and Proteomics. 1865, 3, p. 321-328 8 p.

Research output: Contribution to journalArticle

Open Access
File
Galactokinase
Glutamic Acid
Enzymes
Methionine
Galactosemias
Open Access
File
Open Access
File
Triose-Phosphate Isomerase
Computer Simulation
Mutation
Genes
Amino Acids

IQGAP1 interaction with RHO family proteins revisited: Kinetic andequilibrium evidence for multiple distinct binding sites

Nouri, K., Fansa, E. K., Amin, E., Dvorsky, R., Gremer, L., Willbold, D., Schmitt, L., Timson, D. & Ahmadian, M. R., 4 Nov 2016, In : Journal of Biological Chemistry. 291, 0, p. 26364-26376 13 p.

Research output: Contribution to journalArticle

Open Access
File
GTP Phosphohydrolases
Binding Sites
Proteins
Cell Polarity
Guanosine Triphosphate

Is there a common water-activity limit for the three domains of life?

Stevenson, A., Cray, J. A., Williams, J. P., Santos, R., Sahay, R., Neuenkirchen, N., McClure, C. D., Grant, I. R., Houghton, J. D. R. & Timson, D., 30 Jun 2015, In : ISME Journal. 9, 6, p. 1333-1351 19 p.

Research output: Contribution to journalArticle

Open Access
File

LGR5 receptor promotes cell-cell adhesion in stem cells and colon cancer cells via the IQGAP1 -Rac1 pathway

Carmon, K. S., Xing, G., Yi, J., Wu, L., Thomas, A., Moore, C., Masuho, I., Timson, D., Martemyanov, K. A. & Liu, Q. J., 24 Jul 2017, In : Journal of Biological Chemistry. 292, p. 14989-15001 13 p.

Research output: Contribution to journalArticle

Open Access
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Cell Adhesion
Colonic Neoplasms
Stem Cells
Actins
Catenins

Metabolic Enzymes of Helminth Parasites: Potential as Drug Targets

Timson, D., 31 Dec 2016, In : Current Protein & Peptide Science. 17, 3, p. 280-295 16 p.

Research output: Contribution to journalArticle

Open Access
File

Modulating mobility: a paradigm for protein engineering?

McAuley, M. & Timson, D., 23 Jul 2016, In : Applied Biochemistry and Biotechnology. p. 1-8 8 p.

Research output: Contribution to journalArticle

Open Access
File
Proteins
Ligands
Catalysis
Molecular dynamics
Engineers

Modulation of the mobility of a key region in human galactokinase: Impacts on catalysis and stability

McAuley, M., Huang, M. & Timson, D. J., 19 Sep 2018, In : Bioorganic Chemistry. 81, p. 649-657 9 p.

Research output: Contribution to journalArticle

Open Access
File
Galactokinase
Catalysis
Enzymes
Galactose
Catalytic Domain

Multiplication of microbes below 0.690 water activity: implications for terrestrial and extraterrestrial life

Stevenson, A., Burkhardt, J., Cockell, C. S., Cray, J. A., Dijksterhuis, J., Fox-Powell, M., Kee, T. P., Kminek, G., McGenity, T. J. & Timson, D., 28 Feb 2015, In : Environmental Microbiology. 17, 2, p. 257-277 21 p.

Research output: Contribution to journalArticle

Myosin Va and spermine synthase: partners in exosome transport

Timson, D., 30 Apr 2019, In : Bioscience Reports. 39, 4, p. 1-5 5 p., BSR20190326.

Research output: Contribution to journalArticle

Open Access
File
Spermine Synthase
Exosomes
Myosins
Proteins
Genes

NAD(P)H quinone oxidoreductase (NQO1): An enzyme which needs just enough mobility, in just the right places

Pey, A. L., Megarity, C. F. & Timson, D. J., 3 Jan 2019, In : Bioscience Reports. 39, 1, BSR20180459.

Research output: Contribution to journalArticle

Open Access
File
Flavin-Adenine Dinucleotide
NAD
Oxidoreductases
Dicumarol
Quinones

Natural small molecules as stabilizers and activators of cancer-associated NQO1 polymorphisms

Pey, A. L., Megarity, C. F., Medina-Carmona, E. & Timson, D., 1 Jan 2016, In : Current Drug Targets. 17, 13, p. 1506-1514 9 p.

Research output: Contribution to journalArticle

Open Access
File
Neoplasms
Pharmacology
Quinones
Protein Stability
NAD

Negative cooperativity in NAD(P)H quinone oxidoreductase 1 (NQO1)

Megarity, C. F., Abdel-Aal Bettley, H., Caraher, M. C., Scott, K. A., Whitehead, R. A., Jowitt, T. A., Gutierrez, A., Bryce, R. A., Nolan, K. A., Stratford, I. J. & Timson, D. J., 5 Jun 2019, In : ChemBioChem. 20, 22, p. 2841-2849 9 p.

Research output: Contribution to journalArticle

NAD
Oxidoreductases
Dicumarol
Binding Sites
Proteins

New model for the interaction of IQGAP1 with CDC42 and RAC1

Nouri, K., Timson, D. & Ahmadian, M. R., 19 Jun 2017, In : Small GTPases.

Research output: Contribution to journalArticle

Open Access
File
Proteins
GTP Phosphohydrolases
Adhesion
Polarization