3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes

Elaheh Sirjani, Maciej Migas, Peter Cragg, Marcus Dymond

Research output: Contribution to journalArticle

Abstract

Transparent materials for fused filament fabrication printers are widely available and may be useful for constructing 3D printed devices with applications in UV/VIS spectroscopy. In this study, colourless polylactic acid, HD Glass and T-Glase were evaluated as construction materials for biochemical sensors, which contain immobilised enzymes, for analysis by UV/VIS spectrophotometry. Experiments were conducted on both the native 3D print and after coating with XTC-3D®, a transparent epoxy resin used to improve optical transparency of 3D prints. A combination of HD Glass or T-Glase with XTC-3D®, gave the highest transparency, probably due to the similar refractive indices of these materials. Individual enzymes were immobilised within the 3D prints by coupling the enzymes to tosyl-activated magnetic beads and attracted to the print surface by magnets embedded in the 3D print. A transparent 3D printed device was demonstrated using enzymatic assays of lactose and glucose. This device can be configured to contain up to three separate reaction chambers and features a separate colorimetric analysis chamber. Further studies showed that enzyme assays performed in these 3D printed devices are reproducible, accurate and of comparable sensitivity to the same assays performed in polystyrene cuvettes.
Original languageEnglish
Pages (from-to)101094
JournalAdditive Manufacturing
Volume33
DOIs
Publication statusPublished - 8 Feb 2020

Fingerprint

Immobilized Enzymes
Assays
Transparency
Colorimetric analysis
Enzymes
Epoxy Resins
Glass
Polystyrenes
Spectrophotometry
Lactose
Ultraviolet spectroscopy
Magnets
Refractive index
Glucose
Fabrication
Coatings
Sensors
Experiments

Keywords

  • Fused filament fabrication
  • 3D printed biosensors
  • UV/VIS spectroscopy
  • T-glase
  • HD glass

Cite this

Sirjani, Elaheh ; Migas, Maciej ; Cragg, Peter ; Dymond, Marcus. / 3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes. In: Additive Manufacturing. 2020 ; Vol. 33. pp. 101094.
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3D printed UV/VIS detection systems constructed from transparent filaments and immobilised enzymes. / Sirjani, Elaheh; Migas, Maciej ; Cragg, Peter; Dymond, Marcus.

In: Additive Manufacturing, Vol. 33, 08.02.2020, p. 101094.

Research output: Contribution to journalArticle

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