3D Printed Electrochemical Sensor for Simultaneous Dual Monitoring of Serotonin Overflow and Circular Muscle Contraction

Hisham Bin Hamzah, Oliver Keattch, Mark Yeoman, Derek Covill, Bhavik Patel

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Serotonin (5-HT) is a key signalling molecule within the mucosal epithelium of the intestinal wall and has been shown to be an important modulator of motility. At present no single approach has been established for simultaneous dual measurement of 5-HT overflow and circular muscle contraction. We have developed a 3D printed carbon black / polylactic acid (PLA) electrochemical sensor, which had a geometry suitable for ex vivo measurement in the anorectum. The device was characterised for sensitivity and stability for 5-HT measurements as well as suitability for accurate tracking of anorectal contractions. The 3D printed electrochemical sensor had a linear range in physiological concentrations of 5-HT (1 - 10 µM) present within the intestinal tract and a limit of detection of 540 nM. The sensor was stable for 5-HT measurement following ex vivo tissue measurements. There was a signficant correlation in the amplitude and duration of individual contractions when comparing the measurements using an isometric force transducer and 3D printed electrochemical sensor. Finally, in the presence of 1 µM fluoxetine, the sensor was able to monitor a reduction in contractility as well as an increase in 5-HT overflow as predicted. Overall the 3D printed sensor has the ability to conduct dual simultaneous measurements of 5-HT overflow and contractility. This single device will have significant potential for clinical measurements of anorectum function and signalling, that can direct therapeutic management of patients with bowel disorders.
Original languageEnglish
JournalAnalytical Chemistry
DOIs
Publication statusPublished - 27 Aug 2019

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Muscle Contraction
Serotonin
Soot
Equipment and Supplies
Fluoxetine
Intestinal Mucosa
Transducers
Limit of Detection

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title = "3D Printed Electrochemical Sensor for Simultaneous Dual Monitoring of Serotonin Overflow and Circular Muscle Contraction",
abstract = "Serotonin (5-HT) is a key signalling molecule within the mucosal epithelium of the intestinal wall and has been shown to be an important modulator of motility. At present no single approach has been established for simultaneous dual measurement of 5-HT overflow and circular muscle contraction. We have developed a 3D printed carbon black / polylactic acid (PLA) electrochemical sensor, which had a geometry suitable for ex vivo measurement in the anorectum. The device was characterised for sensitivity and stability for 5-HT measurements as well as suitability for accurate tracking of anorectal contractions. The 3D printed electrochemical sensor had a linear range in physiological concentrations of 5-HT (1 - 10 µM) present within the intestinal tract and a limit of detection of 540 nM. The sensor was stable for 5-HT measurement following ex vivo tissue measurements. There was a signficant correlation in the amplitude and duration of individual contractions when comparing the measurements using an isometric force transducer and 3D printed electrochemical sensor. Finally, in the presence of 1 µM fluoxetine, the sensor was able to monitor a reduction in contractility as well as an increase in 5-HT overflow as predicted. Overall the 3D printed sensor has the ability to conduct dual simultaneous measurements of 5-HT overflow and contractility. This single device will have significant potential for clinical measurements of anorectum function and signalling, that can direct therapeutic management of patients with bowel disorders.",
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3D Printed Electrochemical Sensor for Simultaneous Dual Monitoring of Serotonin Overflow and Circular Muscle Contraction. / Bin Hamzah, Hisham; Keattch, Oliver; Yeoman, Mark; Covill, Derek; Patel, Bhavik.

In: Analytical Chemistry, 27.08.2019.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Covill, Derek

AU - Patel, Bhavik

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N2 - Serotonin (5-HT) is a key signalling molecule within the mucosal epithelium of the intestinal wall and has been shown to be an important modulator of motility. At present no single approach has been established for simultaneous dual measurement of 5-HT overflow and circular muscle contraction. We have developed a 3D printed carbon black / polylactic acid (PLA) electrochemical sensor, which had a geometry suitable for ex vivo measurement in the anorectum. The device was characterised for sensitivity and stability for 5-HT measurements as well as suitability for accurate tracking of anorectal contractions. The 3D printed electrochemical sensor had a linear range in physiological concentrations of 5-HT (1 - 10 µM) present within the intestinal tract and a limit of detection of 540 nM. The sensor was stable for 5-HT measurement following ex vivo tissue measurements. There was a signficant correlation in the amplitude and duration of individual contractions when comparing the measurements using an isometric force transducer and 3D printed electrochemical sensor. Finally, in the presence of 1 µM fluoxetine, the sensor was able to monitor a reduction in contractility as well as an increase in 5-HT overflow as predicted. Overall the 3D printed sensor has the ability to conduct dual simultaneous measurements of 5-HT overflow and contractility. This single device will have significant potential for clinical measurements of anorectum function and signalling, that can direct therapeutic management of patients with bowel disorders.

AB - Serotonin (5-HT) is a key signalling molecule within the mucosal epithelium of the intestinal wall and has been shown to be an important modulator of motility. At present no single approach has been established for simultaneous dual measurement of 5-HT overflow and circular muscle contraction. We have developed a 3D printed carbon black / polylactic acid (PLA) electrochemical sensor, which had a geometry suitable for ex vivo measurement in the anorectum. The device was characterised for sensitivity and stability for 5-HT measurements as well as suitability for accurate tracking of anorectal contractions. The 3D printed electrochemical sensor had a linear range in physiological concentrations of 5-HT (1 - 10 µM) present within the intestinal tract and a limit of detection of 540 nM. The sensor was stable for 5-HT measurement following ex vivo tissue measurements. There was a signficant correlation in the amplitude and duration of individual contractions when comparing the measurements using an isometric force transducer and 3D printed electrochemical sensor. Finally, in the presence of 1 µM fluoxetine, the sensor was able to monitor a reduction in contractility as well as an increase in 5-HT overflow as predicted. Overall the 3D printed sensor has the ability to conduct dual simultaneous measurements of 5-HT overflow and contractility. This single device will have significant potential for clinical measurements of anorectum function and signalling, that can direct therapeutic management of patients with bowel disorders.

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