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Personal profile

Research interests

 

My research interests lie in improving the delivery of drugs and large therapeutic molecules such as DNA and siRNA for the treatment of incurable diseases such as genetic disorders and cancer, through designing and developing novel non-viral gene delivery vectors and understanding their interactions with cellular membranes. A recent study involved the administration of such vectors to the brain via convection enhanced delivery (CED) for the treatment of neurodegenerative diseases.

I have been particularly interested in optimising gene delivery vectors through understanding their structure and physicochemical properties and correlating it to their biological activity, intracellular trafficking, interaction with biological membranes, and ability to overcome cellular barriers.These structure activity relationship studies have been probed using a wide array of techniques such as light scattering, small angle neutron scattering (SANS), neutron reflectivity, circular dichroism, fluorescence spectroscopy, imaging techniques such as confocal and electron microscopy; and biological testing to determine gene transfection, knockdown, toxicity and cell permeability both in-vitro and in-vivo.

Currently, I am interested in understanding the interaction between gene delivery vectors with endosomal membranes (the main barrier to DNA delivery) and designing novel molecules that act as helper lipids to help overcome those barriers and aid DNA entry into the cell nucleus. 

Scholarly biography

I graduated with a first class (Hons) degree in Pharmacy from Liverpool John Moores University, and qualified as a pharmacist after having completed her pre-registration training in both community and hospital pharmacy. I was then awarded the Overseas Research Student Award Scheme (ORSA) from the University of London to undertake a PhD in non-viral gene delivery at King’s College London under the supervision of Prof. Jayne Lawrence.

Following my PhD I was awarded the prestigious CW Maplethorpe Postdoctoral teaching and research fellowship to study the fate of novel lipopolyplex gene delivery vectors in cancer cells, and an EPSRC fellowship to develop novel anionic gene therapy vectors administered to the brain via convection enhanced delivery (CED) for the targeted treatment of neurodegenerative diseases. I then became a lecturer at King’s College London before taking up a senior lectureship position at the University of Brighton.

Approach to teaching

I teach on the MPharm course in the areas of pharmaceutics and biopharmaceutics. My main aim in teaching is to convey a high level of understanding, since this leads to a deeper approach to learning. I therefore use various teaching aids such as animations, short videos and in-class demonstrations to stimulate visual learning and maintain high attention span. My lectures are highly interactive - I like to ask questions and use polling software that allows students to answer questions anonymously with a simple click of a button from their computers or mobile phones. This gives me instant information on the students’ knowledge and understanding, and gives the students instant feedback to the questions. 

Supervisory Interests

I would be interested in supervising undergraduate/Masters/PhD students in the general area of nanomedicines, including the design and formulation of nanoparticles using small drug molecules or large molecules, such as DNA or siRNA for the treatment of cancer or other chronic conditions. I am also interested in understanding cellular barriers and figuring out strategies of overcoming them in order to improve the efficacy of non-viral gene therapy vectors. Skilled, motivated and enthusiastic PhD students are particularly welcome. 

Fingerprint Dive into the research topics where Laila Kudsiova is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

Genes Chemical Compounds
DNA Medicine & Life Sciences
Peptides Medicine & Life Sciences
Small Interfering RNA Medicine & Life Sciences
Lipids Chemical Compounds
Unilamellar Liposomes Chemical Compounds
Nonionic surfactants Chemical Compounds
Transfection Medicine & Life Sciences

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Research Output 2007 2018

The discovery and enhanced properties of trichain lipids in lipopolyplex gene delivery systems

Mohammadi, A., Kudsiova, L., Mustapa, M. F. M., Campbell, F., Vlaho, D., Welser, K., Story, H., Tagalakis, A., Hart, S. L., Barlow, D. J., Tabor, A. B., Lawrence, J. M. & Hailes, H. C., 19 Dec 2018, In : Organic and Biomolecular Chemistry. 17, 4, p. 945-957 13 p.

Research output: Contribution to journalArticle

Open Access
File
Genes
Lipids
DNA
Conformations
Circular dichroism spectroscopy

Trichain cationic lipids: the potential of their lipoplexes for gene delivery

Kudsiova, L., Mohammadi, A., Mustapa, M. F. M., Campbell, F., Welser, K., Vlaho, D., Story, H., Barlow, D. J., Tabor, A. B., Hailes, H. C. & Lawrence, J. M., 16 Oct 2018, In : Biomaterials Science.

Research output: Contribution to journalArticle

Open Access
File
Genes
Lipids
DNA
Photon correlation spectroscopy
Lipid bilayers

Delivery of siRNA using ternary complexes containing branched cationic peptides: the role of peptide sequence, branching and targeting

Kudsiova, L., Welser, K., Campbell, F., Mohammadi, A., Dawson, N., Cui, L., Hailes, H. C., Lawrence, J. M. & Tabor, A. B., 22 Jan 2016, In : Molecular Biosystems. 12, 3, p. 934-951 18 p.

Research output: Contribution to journalArticle

Open Access
File
Small Interfering RNA
Peptides
Lipids
Confocal microscopy
Dynamic light scattering

Manipulating the pH response of 2,3-diaminopropionic acid rich peptides to mediate highly effective gene silencing with low-toxicity

Abbate, V., Liang, W., Patel, J., Lan, Y., Capriotti, L., Iacobucci, V., Bui, T. T., Chaudhuri, P., Kudsiova, L., Vermeer, L. S., Chan, P. F. L., Kong, X., Drake, A. F., Lam, J. K. W., Bansal, S. S. & Mason, A. J., 18 Oct 2013, In : Journal of Controlled Release. 172, 3, p. 929-938 10 p.

Research output: Contribution to journalArticle

Open Access
File
Toxicity
Genes
Peptides
Nucleic Acids
Acidification

Tailoring Nanocomplexes for the Delivery of Plasmid DNA or siRNA: The Role of Cationic Peptide Sequence and Branching

Welser, K., Kudsiova, L., Campbell, F., Mohammadi, A., Dawson, N., Barlow, D. J., Hailes, H. C., Lawrence, J. M. & Tabor, A. B., 1 May 2013, p. 267-267. 1 p.

Research output: Contribution to conferenceAbstract

Small Interfering RNA
Plasmids
Peptides
DNA